| Long name: |
ALEXA FLUOR 647 Conjugated, Cullin 3 Polyclonal Antibody |
| Also known as: |
Anti-Cullin 3 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Cullin 3 |
| Specificity: |
This is a highly specific antibody against Cullin 3 |
| Modification: |
Unmodified |
| Modification Site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
430-480/768 |
| Subcellular location: |
Nucleus |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Cullin 3 |
| Gene ID Number: |
8452 |
| Swiss Prot: |
Q13618 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme, BCR(KLHL12) is involved in ER-Golgi transport by regulating the size of COPII coats, BCR(KLHL3) acts as a regulator of ion transport in the distal nephron, BCR(KLHL42) is involved in ubiquitination of KATNA1, BCR(KLHL9-KLHL13) controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis, BCR(SPOP) is involved in ubiquitination of BMI1/PCGF4, BRMS1, Can also form a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOPL or the heterodimer formed by SPOP and SPOPL, GLI2 and GLI3, H2AFY and DAXX, PubMed:21670212, PubMed:21840486, PubMed:24768539), The BCR(KLHL20) E3 ubiquitin ligase complex is involved in interferon response and anterograde Golgi to endosome transport: it mediates both ubiquitination leading to degradation and 'Lys-33'-linked ubiquitination (PubMed:20389280, The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity), The functional specificity of the BCR complex depends on the BTB domain-containing protein as the substrate recognition component, by mediating ubiquitination of WNK4, thereby playing a key role in collagen export, these complexes have lower ubiquitin ligase activity, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B), Core component of multiple cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the Cullin 3 Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
Cullin 3 |
| Short name: |
Cullin 3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, Cullin 3 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2553 |
| Gene: |
CUL3 |
More about : CUL3 |
| Long gene name: |
cullin 3 |
| Locus: |
2q36, 2 |
| Discovery year: |
1998-10-29 |
| GenBank acession: |
U58089 |
| Entrez gene record: |
8452 |
| Pubmed identfication: |
8681378 17192413 |
| Classification: |
Cullins |
| Havana BLAST/BLAT: |
OTTHUMG00000133167 |