| Long name: |
Biotin Conjugated, ATG4D Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ATG4D |
| Specificity: |
This is a highly specific antibody against ATG4D |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
425-474/474 |
| Subcellular location: |
Mitochondrion, Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human ATG4D |
| Gene ID number: |
84971 |
| Swiss Prot: |
Q86TL0 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, Cysteine protease ATG4D, Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, Has also an activity of delipidating enzyme for the PE-conjugated forms, ROS, The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis, mitophagy and cell viability, to reveal a C-terminal glycine, which is necessary for autophagy, Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
APG4-D |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ATG4D Conjugated |
| Short name: |
Conjugated, ATG4D Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, ATG4D (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
20789 |
| Gene: |
ATG4D |
More about : ATG4D |
| Long gene name: |
autophagy related 4D cysteine peptidase |
| Synonyms gene: |
AUTL4 APG4D |
| Synonyms gene name: |
AUT-like 4, cerevisiae) APG4 autophagy 4 homolog D (S, cerevisiae) ATG4 autophagy related 4 homolog D (S, cerevisiae) autophagy related 4D, cysteine endopeptidase (S, cysteine peptidase |
| Locus: |
19p13, 2 |
| Discovery year: |
2003-04-11 |
| GenBank acession: |
AJ312332 |
| Entrez gene record: |
84971 |
| Pubmed identfication: |
12446702 |
| RefSeq identity: |
NM_032885 |
| Classification: |
Autophagy related |
| Havana BLAST/BLAT: |
OTTHUMG00000180582 |