| Long name: |
FITC Conjugated, PABP (Methyl Arg455/460) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PABP Methyl Arg455/460 |
| Specificity: |
This is a highly specific antibody against PABP Methyl Arg455/460 |
| Modification: |
Methylation |
| Modification site: |
Methyl Arg455/460 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
440-480/636 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic metylpeptide derived from human PABP around metylation site of Arg455/Arg460 |
| Gene ID number: |
26986 |
| Swiss Prot: |
P11940 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo, Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain, Involved in regulation of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons, Involved in translationally coupled mRNA turnover, Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2, May be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing, for the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed, including that of its own transcript, Binds the poly(A) tail of mRNA |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
PABP, PABP-1, PABP1, PABPC1, PABPC2, PABPL1, Poly(A)-binding protein 1, Polyadenylate-binding protein 1, PAB1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Description: |
All three forms are highly reactive and rarely observed, CH3, In , It is a very stable group in most molecules, Such hydrocarbon , The anion has eight valence electrons, While the methyl group is usually part of a larger , containing one , it can be found on its own in any of three forms , the group is often , the radical seven and the cation six, , A , Me, abbreviated , alkyl , anion, atom , atoms &mdash, bonded , carbon , cation , derived from , formulas, groups occur in many , hydrogen , is an , methane, methyl group , molecule, or , organic compounds, radical, to three |
| Gene target: |
PABP (Methyl Arg455/460) Conjugated |
| Short name: |
FITC Conjugated, PABP (Methyl Arg455/460) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, PABP (Methyl Arg455/460) (Antibody to) |
| Alternative technique: |
antibodies |