| Long name: |
FITC Conjugated, eIF4E Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
e4E |
| Specificity: |
This is a highly specific antibody against e4E |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human eIF4E |
| Gene ID number: |
1977 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Phosphorylation also enhances the interaction of eIF4E with eIF4G, Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA, Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription, eIF4E also influences the overall rate of translation, eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, eIF4E phosphorylation is correlated with increased translational rate in a number of cell types, which form a complex known as eIF4F, eIF4E |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CBP, CBP, E 4F, E4E1, E4EL1, E4F, Eukaryotic Translation Initiation Factor 4 E, Eukaryotic translation initiation factor 4E like 1, Messanger RNA Cap Binding Protein e 4E, e 4E, e 4E, e 4F 25 kDa subunit, e 4F 25 kDa subunit, e-4E, E4F |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
eIF4E Conjugated |
| Short name: |
FITC Conjugated, eIF4E Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, eIF4E (Antibody to) |
| Alternative technique: |
antibodies |