| Long name: |
Biotin Conjugated, eIF4E (Ser209) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
eIF4E Ser209 |
| Specificity: |
This is a highly specific antibody against eIF4E Ser209 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser209 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
200-217/217 |
| Subcellular location: |
Helical |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 [SG(p-S)TT] |
| Gene ID number: |
1977 |
| Swiss Prot: |
P06730 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression, In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap, Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
AUTS19, EIF4E1, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4E, eIF-4F 25 kDa subunit, eIF4E, mRNA cap-binding protein, CBP |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
eIF4E (Ser209) Conjugated |
| Short name: |
Conjugated, eIF4E (Ser209) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, eIF4E (Ser209) (Antibody to) |
| Alternative technique: |
antibodies |