| Long name: |
FITC Conjugated, KAP1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
KAP1 |
| Specificity: |
This is a highly specific antibody against KAP1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
650-700/835 |
| Subcellular location: |
Nucleus |
| Source: |
KLH conjugated synthetic peptide derived from human KAP1/TIF1b |
| Gene ID number: |
10155 |
| Swiss Prot: |
Q13263 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also corepressor for ERBB4, Also specifically sumoylates IRF7, Enhances transcriptional repression by coordinating the increase in H3K9me, Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger, Important regulator of CDKN1A/p21(CIP1), Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation, May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1, May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1, Mediates gene silencing by recruiting CHD3, Mediates the nuclear localization of KOX1, Recruitment of SETDB1 induces heterochromatinization, Ubiquitinates p53/TP53 leading to its proteosomal degradation, ZNF268 and ZNF300 transcription factors, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes, and possibly MAGEA3 and MAGEA6, respectively) and the disposition of HP1 proteins to silence gene expression, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, the function is enhanced by MAGEC2 and MAGEA2, thereby inhibiting its transactivation activity, Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
TIF1B KAP1 TF1B RNF96 PPP1R157 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
KAP1 Conjugated |
| Short name: |
FITC Conjugated, KAP1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, KAP1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
16384 |
| Gene: |
TRIM28 |
More about : TRIM28 |
| Long gene name: |
tripartite motif containing 28 |
| Synonyms gene name: |
tripartite motif-containing 28 |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 157 |
| Locus: |
19q13, 4 |
| Discovery year: |
2001-08-10 |
| Entrez gene record: |
10155 |
| Pubmed identfication: |
11331580 11226167 |
| RefSeq identity: |
NM_005762 |
| Classification: |
Tripartite motif containing PHD finger proteins Ring finger proteins Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000183546 |