| Long name: |
FITC Conjugated, mTOR (Ser2448) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
mTOR Ser2448 |
| Specificity: |
This phosphorylation site is homologous across the listed species, This is a highly specific antibody against mTOR Ser2448 |
| Modification: |
Phosphorylation |
| Modification site: |
Ser2448 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
2420-2470/2549 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Ser2448 |
| Gene ID number: |
2475 |
| Swiss Prot: |
P42345 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis, Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2), In parallel to protein synthesis, MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins, Moreover, Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor, Stimulates the pyrimidine biosynthesis pathway, This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E), To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A, Under nutrient sufficiency, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1, an allosteric activator of CAD at a later step in synthesis, and delayed regulation, and the inhibitor of translation initiation PDCD4, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, disrupting the interaction with AMPK and preventing activation of ULK1, energy and stress signals, eukaryotic translation initiation factor EIF4B, growth and survival in response to hormones, growth factors, mTORC1 also negatively regulates autophagy through phosphorylation of ULK1, nutrients, phosphorylates ULK1 at 'Ser-758', phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, this function is dependent on the mTORC1 complex, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), Serine/threonine protein kinase which is a central regulator of cellular metabolism |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, RAFT1, RAPT1, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Serine/threonine-protein kinase mTOR, mTOR, FRAP |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
mTOR (Ser2448) Conjugated |
| Short name: |
FITC Conjugated, mTOR (Ser2448) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Mammalian target on rapamycin (Ser2448) (Antibody to) |
| Alternative technique: |
antibodies |