| Long name: |
ALEXA FLUOR 647 Conjugated, mTOR (Ser2448) Polyclonal Antibody |
| Also known as: |
Anti-mTOR Ser2448 PAb ALEXA FLUOR 647 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
647, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
mTOR Ser2448 |
| Specificity: |
This is a highly specific antibody against mTOR Ser2448 |
| Modification: |
Phosphorylation |
| Modification Site: |
Ser2448 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
2420-2470/2549 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human mTOR around the phosphorylation site of Ser2448 |
| Gene ID Number: |
2475 |
| Swiss Prot: |
P42345 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis, Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2), In parallel to protein synthesis, MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins, Moreover, Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor, Stimulates the pyrimidine biosynthesis pathway, This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E), To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A, Under nutrient sufficiency, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1, an allosteric activator of CAD at a later step in synthesis, and delayed regulation, and the inhibitor of translation initiation PDCD4, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, disrupting the interaction with AMPK and preventing activation of ULK1, energy and stress signals, eukaryotic translation initiation factor EIF4B, growth and survival in response to hormones, growth factors, mTORC1 also negatively regulates autophagy through phosphorylation of ULK1, nutrients, phosphorylates ULK1 at 'Ser-758', phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, this function is dependent on the mTORC1 complex, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), Serine/threonine protein kinase which is a central regulator of cellular metabolism |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
650nm/665nm |
| Synonyms: |
FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP1, FRAP2, Mammalian target of rapamycin, Mechanistic target of rapamycin, RAFT1, RAPT1, Rapamycin and FKBP12 target 1, Rapamycin target protein 1, Serine/threonine-protein kinase mTOR, mTOR, FRAP |
| Properties: |
ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode, ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 633 is a practical alternative to APC as well as Cy5, Bioss Primary Conjugated Antibodies, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is detected in the FL4 detector of the core's upgraded 2-laser FACScans, Like other Alexa Fluor dyes, exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy, the mTOR (Ser2448) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
ALEXA FLUOR 647, Alexa Fluor |
| Gene target: |
mTOR (Ser2448) |
| Short name: |
mTOR (Ser2448) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 647 |
| Alternative name: |
ALEXA FLUOR 647, Mammalian target on rapamycin (Ser2448) (Antibody to) |
| Alternative technique: |
antibodies |