| Long name: |
FITC Conjugated, A Raf (Tyr301 + Tyr302) Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
A Raf Tyr301 + Tyr302 |
| Specificity: |
This is a highly specific antibody against A Raf Tyr301 + Tyr302 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr301 + Tyr302 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synstic phosphopeptide derived from human A Raf around the phosphorylation site of Tyr301/302 [G(p-Y)(p-Y)WE] |
| Gene ID number: |
369 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
14-3-3 is also known to be an activator of RAFs, A RAF is expressed at a very high concentration in urogenital and kidney tissues, Activated RAF directly phosphorylates and activates MEK, RAF 1 and B RAF, RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain, These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway, This domain is conserved in A RAF and B RAF, The RAF family of signaling proteins has three isoforms: A RAF |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
A Raf proto oncogene serine/threonine protein kinase, A raf 1, ARAF, ARAF 1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, Oncogene Araf1, Oncogene PKS2, PKS, PKS 2, PKS2, Proto oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, p-A RAF phospho Y301 + Y302, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 murine sarcoma 3611 virus, A RAF phospho Y301 + Y302 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
A Raf (Tyr301 + Tyr302) Conjugated |
| Short name: |
FITC Conjugated, A Raf (Tyr301 + Tyr302) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, A Raf (Tyr301 and Tyr302) (Antibody to) |
| Alternative technique: |
antibodies |