| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
A Raf Tyr301 + Tyr302 |
| Specificity: |
This antibody reacts specifically with A Raf (Tyr301 + Tyr302) |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr301 + Tyr302 |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synstic phosphopeptide derived from human A Raf around the phosphorylation site of Tyr301/302 [G(p-Y)(p-Y)WE] |
| Gene ID: |
369 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
14-3-3 is also known to be an activator of RAFs, A RAF is expressed at a very high concentration in urogenital and kidney tissues, Activated RAF directly phosphorylates and activates MEK, RAF 1 and B RAF, RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain, These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway, This domain is conserved in A RAF and B RAF, The RAF family of signaling proteins has three isoforms: A RAF |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
A Raf proto oncogene serine/threonine protein kinase, A raf 1, ARAF, ARAF 1, ARAF1, ARAF_HUMAN, ARaf proto oncogene serine/threonine protein kinase, Oncogene Araf1, Oncogene PKS2, PKS, PKS 2, PKS2, Proto oncogene Pks, RAFA 1, RAFA1, Ras binding protein DA Raf, p-A RAF phospho Y301 + Y302, v raf murine sarcoma 3611 viral oncogene homolog, v raf murine sarcoma 3611 viral oncogene homolog 1, v raf oncogene homolog 1 murine sarcoma 3611 virus, A RAF phospho Y301 + Y302 |
| Also known as: |
A Raf (Tyr301 + Tyr302) Antibody |
| Other name: |
Anti-A Raf (Tyr301 + Tyr302) |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
A Raf (Tyr301 + Tyr302) |
| Short name: |
Anti-A Raf (Tyr301 + Tyr302) Fluor 594 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-A Raf (Tyr301 and Tyr302) |