| Long name: |
Biotin Conjugated, PKR (Thr446) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
PKR Thr446 |
| Specificity: |
This is a highly specific antibody against PKR Thr446 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr446 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
420-470/551 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human PKR around the phosphorylation site of Thr446 |
| Gene ID number: |
5610 |
| Swiss Prot: |
P19525 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs, Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6, Also phosphorylates other substrates including p53/TP53, Can act as both a positive and negative regulator of the insulin signaling pathway (ISP), DHX9, Either as an adapter protein and/or via its kinase activity, Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), ILF3, IRF1, IRS1 and the HHV-1 viral protein US11, In addition to serine/threonine-protein kinase activity, Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, PPP2R5A, STAT1, STAT3, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, apoptosis, can regulate various signaling pathways (p38 MAP kinase, cell proliferation and differentiation, facilitating its ubiquitination and proteosomal degradation, hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2), IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
PKR EIF2AK1 PPP1R83 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
PKR (Thr446) Conjugated |
| Short name: |
Conjugated, PKR (Thr446) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, PKR (Thr446) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9437 |
| Gene: |
EIF2AK2 |
More about : EIF2AK2 |
| Long gene name: |
eukaryotic translation initiation factor 2 alpha kinase 2 |
| Synonyms gene: |
PRKR |
| Synonyms gene name: |
interferon-inducible double stranded RNA dependent , protein kinase |
| Synonyms name: |
protein phosphatase 1, regulatory subunit 83 |
| Locus: |
2p22, 2 |
| Discovery year: |
1992-12-04 |
| GenBank acession: |
BC057805 |
| Entrez gene record: |
5610 |
| Pubmed identfication: |
1351683 |
| RefSeq identity: |
NM_002759 |
| Classification: |
Protein phosphatase 1 regulatory subunits |
| Havana BLAST/BLAT: |
OTTHUMG00000100962 |