| Long name: |
Cy3 Conjugated, MAPKAPK2 (Thr222) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
cy3 conjugation kit |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MAPKAPK2 Thr222 |
| Specificity: |
This is a highly specific antibody against MAPKAPK2 Thr222 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr222 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
192-230/400 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Thr222 |
| Gene ID number: |
9261 |
| Swiss Prot: |
P49137 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Bovine, Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, CDC25B, CDC25C, DNA damage response and transcriptional regulation, ELAVL1, Following stress, HNRNPA, HNRNPA, HSF1, HSP27/HSPB1, Hyd-X-R-X(2)-S, Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3, KRT18, KRT2, LIMK1, LSP1, Mediates phosphorylation of HSP27/HSPB1 in response to stress, PABPC1, PABPC1 and TTP/ZFP36, PARN, PDE4A, Phosphorylates ALOX5, Phosphorylates serine in the peptide sequence, Phosphorylation of TTP/ZFP36, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36, a major post-transcriptional regulator of TNF, cell cycle control, cell migration, chromatin remodeling, endocytosis, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively, leading to phosphorylation of substrates, leading to regulate the stability and translation of TNF and IL6 mRNAs, leading to stabilize GADD45A mRNA, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA and PARN, reorganization of the cytoskeleton, where Hyd is a large hydrophobic residue, Stress-activated serine/threonine-protein kinase involved in cytokines production |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
512, 550nm/570, 615nm |
| Properties: |
1 of the MAPKAPK2 (Thr222) Antibody, Conjugated can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC, Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser, This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
MAPKAPK2 (Thr222) Conjugated |
| Short name: |
Conjugated, MAPKAPK2 (Thr222) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Cy3 |
| Alternative name: |
cyanine 3 coupled, MAPKAPK2 (Thr222) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6887 |
| Gene: |
MAPKAPK2 |
More about : MAPKAPK2 |
| Long gene name: |
mitogen-activated protein kinase-activated protein kinase 2 |
| Locus: |
1q32, 1 |
| Discovery year: |
1999-03-26 |
| GenBank acession: |
U12779 |
| Entrez gene record: |
9261 |
| Pubmed identfication: |
8179591 8280084 |
| RefSeq identity: |
NM_004759 |
| Classification: |
Mitogen-activated protein kinase-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000036342 |