| Long name: |
ALEXA FLUOR 488 Conjugated, MAPKAPK2 (Thr222) Polyclonal Antibody |
| Also known as: |
Anti-MAPKAPK2 Thr222 PAb ALEXA FLUOR 488 |
| Category: |
Conjugated Primary Antibodies |
| Conjugated with: |
488, ALEXA FLUOR® |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
MAPKAPK2 Thr222 |
| Specificity: |
This is a highly specific antibody against MAPKAPK2 Thr222 |
| Modification: |
Phosphorylation |
| Modification Site: |
Thr222 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen range: |
192-230/400 |
| Subcellular location: |
Nucleus, Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human MAPKAPK2 around the phosphorylation site of Thr222 |
| Gene ID Number: |
9261 |
| Swiss Prot: |
P49137 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Bovine, Mouse, Rat, Human |
| Cross-reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the antigen: |
Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, CDC25B, CDC25C, DNA damage response and transcriptional regulation, ELAVL1, Following stress, HNRNPA, HNRNPA, HSF1, HSP27/HSPB1, Hyd-X-R-X(2)-S, Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3, KRT18, KRT2, LIMK1, LSP1, Mediates phosphorylation of HSP27/HSPB1 in response to stress, PABPC1, PABPC1 and TTP/ZFP36, PARN, PDE4A, Phosphorylates ALOX5, Phosphorylates serine in the peptide sequence, Phosphorylation of TTP/ZFP36, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36, a major post-transcriptional regulator of TNF, cell cycle control, cell migration, chromatin remodeling, endocytosis, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively, leading to phosphorylation of substrates, leading to regulate the stability and translation of TNF and IL6 mRNAs, leading to stabilize GADD45A mRNA, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA and PARN, reorganization of the cytoskeleton, where Hyd is a large hydrophobic residue, Stress-activated serine/threonine-protein kinase involved in cytokines production |
| Purification: |
Purified by Protein A |
| Storage conditions: |
50% glycerol and 0, Keep refrigerated at 2 to 8 degrees Celcius for up to one year, 09% sodium azide, Store this antibody in aqueous buffered solution containing 1% BSA |
| Excitation emission: |
499nm/519nm |
| Properties: |
ALEXA FLUOR they should be stored frozen at - 24°, Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), Also Alexa Fluor 488 is pH stable, As a result of this photo stability, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies, It is distinguished in the FL1 of a FACS-Calibur or FACScan, has a very high photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry, yet the MAPKAPK2 (Thr222) Antibody, C, C for long term storage and for short term at + 5°, For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
MAPKAPK2 (Thr222) |
| Short name: |
MAPKAPK2 (Thr222) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Label: |
ALEXA FLUOR 488 |
| Alternative name: |
ALEXA FLUOR 488, MAPKAPK2 (Thr222) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6887 |
| Gene: |
MAPKAPK2 |
More about : MAPKAPK2 |
| Long gene name: |
mitogen-activated protein kinase-activated protein kinase 2 |
| Locus: |
1q32, 1 |
| Discovery year: |
1999-03-26 |
| GenBank acession: |
U12779 |
| Entrez gene record: |
9261 |
| Pubmed identfication: |
8179591 8280084 |
| RefSeq identity: |
NM_004759 |
| Classification: |
Mitogen-activated protein kinase-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000036342 |