| Long name: |
HRP Conjugated, BTK (Tyr223) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
HRP Conjugated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
BTK Tyr223 |
| Specificity: |
This phosphorylation site is homologous in the listed cross reactive species at the specified location, This is a highly specific antibody against BTK Tyr223 |
| Modification: |
Phosphorylation |
| Modification site: |
Tyr223 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
190-240/659 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Btk around the phosphorylation site of Tyr223 [AL(p-Y)DY] |
| Gene ID number: |
695 |
| Swiss Prot: |
Q06187 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
ARID3A and NFAT are other transcriptional target of BTK, After BCR engagement and activation at the plasma membrane, BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways, BTK has a dual role in the regulation of apoptosis, BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B, BTK is required for the formation of functional ARID3A DNA-binding complexes, BTK plays also a critical role in transcription regulation, Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation, Especially, GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression, Induces the activity of NF-kappa-B, PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK, Plays an important role in the function of immune cells of innate as well as adaptive immunity, The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense, There is however no evidence that BTK itself binds directly to DNA, Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR, Within the TLR pathway, as a component of the Toll-like receptors (TLR) pathway, differentiation and signaling, followed by activation of the protein kinase C (PKC) family members, igniting the downstream signaling pathway through calcium mobilization, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation, is a critical molecule in regulating TLR9 activation in splenic B-cells, phosphorylates PLCG2 at several sites, which is involved in regulating the expression of hundreds of genes, Non-receptor tyrosine kinase indispensable for B lymphocyte development |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% Gentamicin, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
ATK XLA PSCTK1 |
| Properties: |
Also monoclonal HRP conjugates are often used for Western Blot, HRP conjugates are often primary rabbit polyclonal antibodies couples to the , found in the roots of , (HRP), C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°, enzyme , horseradish, horseradish peroxidase |
| Gene target: |
BTK (Tyr223) Conjugated |
| Short name: |
Conjugated, BTK (Tyr223) Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
HRP |
| Alternative name: |
horseradish peroxidase coupled, Bruton agammaglobulinemia tyrosine kinase (Tyr223) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
AGMX1 and AT and ATK and BPK and IMD1 and PSCTK1 and XLA, BTK and IDBG-633447 and ENSBTAG00000019250 and 533459, BTK and IDBG-79651 and ENSG00000010671 and 695, Btk and IDBG-171427 and ENSMUSG00000031264 and 12229, DNA-templated and biological process this GO :0006468 and protein phosphorylation and biological process this GO :0007249 and I-kappaB kinase/NF-kappaB signaling and biological process this GO :0007498 and mesoderm development and biological process this GO :0010033 and response to organic substance and biological process this GO :0016772 and transferase activity, nuclei, phosphatidylinositol-3, this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002250 and adaptive immune response and biological process this GO :0002553 and histamine secretion by mast cell and biological process this GO :0002721 and regulation of B cell cytokine production and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002902 and regulation of B cell apoptotic process and biological process this GO :0004672 and protein kinase activity and molecular function this GO :0004713 and protein tyrosine kinase activity and molecular function this GO :0004715 and non-membrane spanning protein tyrosine kinase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005524 and ATP binding and molecular function this GO :0005547 and phosphatidylinositol-3, this GO :0004672 : protein kinase activity, this GO :0004672 : protein kinase activity and also this GO :0004713 : protein tyrosine kinase activity and also this GO :0004715 : non-membrane spanning protein tyrosine kinase activity and also this GO :0005515 : protein binding and also this GO :0005524 : ATP binding and also this GO :0005547 : phosphatidylinositol-3, this GO :0004713 : protein tyrosine kinase activity, this GO :0004715 : non-membrane spanning protein tyrosine kinase activity, this GO :0005515 : protein binding, this GO :0005524 : ATP binding, this GO :0005547 : phosphatidylinositol-3, this GO :0016772 : transferase activity, this GO :0042802 : identical protein binding, this GO :0046872 : metal ion binding, transferring phosphorus-containing groups, transferring phosphorus-containing groups and also this GO :0042802 : identical protein binding and also this GO :0046872 : metal ion binding, transferring phosphorus-containing groups and molecular function this GO :0018108 and peptidyl-tyrosine phosphorylation and biological process this GO :0019722 and calcium-mediated signaling and biological process this GO :0031410 and cytoplasmic vesicle and cellular component this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034614 and cellular response to reactive oxygen species and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0042113 and B cell activation and biological process this GO :0042629 and mast cell granule and cellular component this GO :0042802 and identical protein binding and molecular function this GO :0043231 and intracellular membrane-bounded organelle and cellular component this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045579 and positive regulation of B cell differentiation and biological process this GO :0046777 and protein autophosphorylation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0048469 and cell maturation and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050853 and B cell receptor signaling pathway and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0071226 and cellular response to molecule of fungal origin and biological process this GO :0097190 and apoptotic signaling pathway and biological process, 4, 4, 4, 5-trisphosphate binding, 5-trisphosphate binding and also this GO :0016772 : transferase activity, 5-trisphosphate binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006351 and transcription, Bruton agammaglobulinemia tyrosine kinase |
| Identity: |
1133 |
| Gene: |
BTK |
More about : BTK |
| Long gene name: |
Bruton tyrosine kinase |
| Synonyms gene: |
AGMX1 IMD1 |
| Synonyms gene name: |
Bruton agammaglobulinemia tyrosine kinase |
| Synonyms name: |
Bruton', s tyrosine kinase |
| Locus: |
Xq22, 1 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
AK057105 |
| Entrez gene record: |
695 |
| Pubmed identfication: |
8380905 |
| RefSeq identity: |
NM_000061 |
| Classification: |
Pleckstrin homology domain containing SH2 domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000022022 |
| Locus Specific Databases: |
BTK base: Mutation registry for X-linked agammaglobulinemia Mental Retardation database LRG_128 |