| Long name: |
FITC Conjugated, Bcl-2 (Thr56) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Bcl-2 Thr56 |
| Specificity: |
This is a highly specific antibody against Bcl-2 Thr56 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr56 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human Bcl-2 around the phosphorylation site of Thr56 |
| Gene ID number: |
596 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Also interacts with APAF1, BAD, BAK and BclX(L), BBC3, BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1), BCL2L1 and BNIPL, Constitutive expression of BCL2, Heterodimerization with BAX requires intact BH1 and BH2 domains, It appears to function in a feedback loop system with caspases, It can form homodimers, It regulates cell death by controlling the mitochondrial membrane permeability, RAF1, TP53BP2, Two transcript variants (alpha and beta) produced by alternate splicing, and heterodimers with BAX, and is necessary for anti-apoptotic activity, differ in their C-terminal ends, is thought to be the cause of follicular lymphoma, such as in the case of translocation of BCL2 to Ig heavy chain locus, BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes, BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
2, AW986256, Apoptosis regulator Bcl 2, Apoptosis regulator Bcl2, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, B cell lymphoma 2, B-cell, BCL2 protein, BCL2_HUMAN, Bcl 2, Bcl-2, Bcl2, Bcl2 phospho Thr56, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, Oncogene B-cell leukemia 2, p-Bcl2 phospho T56, Bcl2 phospho T56 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Bcl-2 (Thr56) Conjugated |
| Short name: |
FITC Conjugated, Bcl-2 (Thr56) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Bcl-2 (Thr56) (Antibody to) |
| Alternative technique: |
antibodies |