Long name: |
FITC Conjugated, Thrombin heavy chain Polyclonal Antibody |
Category: |
Conjugated Primary Antibodies |
Conjugation: |
Anti-FITC Antibody |
Host Organism: |
Rabbit (Oryctolagus cuniculus) |
Target Antigen: |
Thrombin heavy chain |
Specificity: |
This is a highly specific antibody against Thrombin heavy chain |
Modification: |
Unmodified |
Modification site: |
None |
Clonality: |
Polyclonal |
Clone: |
Polyclonal antibody |
Concentration: |
1ug per 1ul |
Source: |
KLH conjugated synthetic peptide derived from human Thrombin heavy chain |
Gene ID number: |
2147 |
Tested applications: |
IF(IHC-P) |
Recommended dilutions: |
IF(IHC-P)(1:50-200) |
Crossreactivity: |
Mouse, Rat, Human |
Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
Antigen background: |
Glycosaminoglycan binding to exosite II of thrombin plays a major role in switching off the procoagulant functions of thrombin by mediating its irreversible inhibition by circulating serpins and by its binding to the endothelial cell surface receptor thrombomodulin, The ability of thrombin to specifically recognize a wide range of substrates derives from interactions which occur outside of the active site of thrombin, Thrombin possesses two anion binding exosites which mediate many of its interactions with cofactors and substrates, and although many structures of thrombin have been solved, few such interactions have been described in molecular detail, Thrombin is the final protease in the blood coagulation cascade and serves both pro- and anticoagulant functions through the cleavage of several targets |
Purification method: |
This antibody was purified via Protein A |
Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
Excitation Emission: |
494nm/518nm |
Synonyms: |
Cf-2, Cf2, Coagulation factor II, Coagulation factor II precursor, F 2, F2, F2, FII, Factor II, Factor-II, PT, Prothrombin, THRB, THRB_HUMAN, Thrombin, Thrombin heavy chain, coagulation factor II thrombin, prothrombin, prothrombin B-chain, serine protease, coagulation factor II |
Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
Gene target: |
Thrombin heavy chain Conjugated |
Short name: |
FITC Conjugated, Thrombin heavy chain Antibody |
Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
Label: |
FITC |
Alternative name: |
fluorecein coupled, Thrombin heavy epitope (Antibody to) |
Alternative technique: |
antibodies |