| Long name: |
FITC Conjugated, HCST Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
HCST |
| Specificity: |
This is a highly specific antibody against HCST |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
1-50/93 |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human HCST |
| Gene ID number: |
10870 |
| Swiss Prot: |
Q9UBK5 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Both PIK3R1 and GRB2 are required for full KLRK1-HCST-mediated activation and ultimate killing of target cells, Functions as docking site for PI3-kinase PIK3R1 and GRB2, In NK cells, In T-cells, Interaction of ULBPs with KLRK1-HCST triggers calcium mobilization and activation of the PIK3R1, KLRK1-HCST receptor plays a role in immune surveillance against tumors and is required for cytolysis of tumors cells, KLRK1-HCST signaling directly induces cytotoxicity and enhances cytokine production initiated via DAP12/TYROBP-associated receptors, MAP2K/ERK, and JAK2/STAT5 signaling pathways, and UL16-binding proteins (ULBPs), indeed, it provides primarily costimulation for TCR-induced signals, melanoma cells that do not express KLRK1 ligands escape from immune surveillance mediated by NK cells, these ligands are up-regulated by stress conditions and pathological state such as viral infection and tumor transformation, this receptor plays a major role in triggering cytotoxicity against target cells expressing cell surface ligands such as MHC class I chain-related MICA and MICB, Transmembrane adapter protein which associates with KLRK1 to form an activation receptor KLRK1-HCST in lymphoid and myeloid cells |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
CMPST hCST |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
HCST Conjugated |
| Short name: |
FITC Conjugated, HCST Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, HCST (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
11021 |
| Gene: |
SLC35A1 |
More about : SLC35A1 |
| Long gene name: |
solute carrier family 35 member A1 |
| Synonyms gene name: |
member 1 solute carrier family 35 (CMP-sialic acid transporter), member A1 , solute carrier family 35 (UDP-galactose transporter) |
| Locus: |
6q15 |
| Discovery year: |
1999-10-05 |
| GenBank acession: |
D87969 |
| Entrez gene record: |
10559 |
| Pubmed identfication: |
9010752 9644260 |
| Classification: |
Solute carriers |
| Havana BLAST/BLAT: |
OTTHUMG00000015177 |