| Long name: |
FITC Conjugated, ERK1 (T203/Y205) + ERK2 (T183/Y185) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ERK1 (Thr203/Tyr205) + ERK2 (Thr183/Tyr185) |
| Specificity: |
This is a highly specific antibody against ERK1 (Thr203/Tyr205) + ERK2 (Thr183/Tyr185) |
| Modification: |
Phosphorylation |
| Modification site: |
Thr183/Tyr 185 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
160-200/358 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic phosphopeptide derived from mouse ERK2 around the phosphorylation site of Thr183 and Tyr 185 |
| Gene ID number: |
26413 |
| Swiss Prot: |
P63085 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Bovine, Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Binds to a [GC]AAA[GC] consensus sequence, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1, Repress the expression of interferon gamma-induced genes, Seems to bind to the promoter of CCL5, Transcriptional activity is independent of kinase activity (By similarity), Acts as a transcriptional repressor |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
ERK ERK2 p41mapk MAPK2 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ERK1 (T203/Y205) + ERK2 (T183/Y185) Conjugated |
| Short name: |
FITC Conjugated, ERK1 (T203/Y205) + ERK2 (T183/Y185) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ERK1 (T203/Y205) and ERK2 (T183/Y185) (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
6871 |
| Gene: |
MAPK1 |
More about : MAPK1 |
| Long gene name: |
mitogen-activated protein kinase 1 |
| Synonyms gene: |
PRKM2 PRKM1 |
| Locus: |
22q11, 22 |
| Discovery year: |
1993-11-05 |
| GenBank acession: |
M84489 |
| Entrez gene record: |
5594 |
| Classification: |
Mitogen-activated protein kinases |
| Havana BLAST/BLAT: |
OTTHUMG00000030508 |
| Locus Specific Databases: |
LRG_786 |