| Long name: |
FITC Conjugated, NOSTRIN Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
NOSTRIN |
| Specificity: |
This is a highly specific antibody against NOSTRIN |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human NOSTRIN |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
It consists of an N-terminal Cdc15 domain with an FCH (Fes/CIP homology) region, It functions as an adaptor protein binding to caveolin-1 via an internal domain and NOS3 via its SH3 domain, NOSTRIN also interacts with Dynamin and N-WASP via its SH3 domain, NOSTRIN is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, NOSTRIN typically exists as a trimer, The NOS3 protein is responsible for the production of nitric oxide (NO), The translocation of NOS3 from the plasma membrane to intracellular vesicle-like structures diminishes NO production, a potent mediator in various biological processes, also known as endothelial nitric oxide synthase traffic inducer, forming a ternary complex which facilitates caveolar transport of NOS3, is a member of the Pombe Cdc15 homology (PCH) family of proteins, kidney and heart, lung, two coiled coil domains and a C-terminal SH3 domain, NOSTRIN (nitric oxide synthase trafficker isoform 1) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
MGC20702 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
NOSTRIN Conjugated |
| Short name: |
FITC Conjugated, NOSTRIN Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, nitric oxide synthase trafficker (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, DNA-templated and biological process this GO :0046209 and nitric oxide metabolic process and biological process this GO :0050999 and regulation of nitric-oxide synthase activity and biological process, NOSTRIN and IDBG-74325 and ENSG00000163072 and 115677, Nostrin and IDBG-175946 and ENSMUSG00000034738 and 329416, nuclei, protein binding, this GO :0003677 and DNA binding and molecular function this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005856 and cytoskeleton and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006897 and endocytosis and biological process this GO :0007165 and signal transduction and biological process this GO :0030666 and endocytic vesicle membrane and cellular component this GO :0044281 and small molecule metabolic process and biological process this GO :0045892 and negative regulation of transcription, this GO :0003677 : DNA binding, this GO :0003677 : DNA binding and also this GO :0005515 : protein binding, this GO :0005515 : protein binding, 17865 and IDBG-641032 and ENSBTAG00000010362 and 521834, nitric oxide synthase trafficker |
| Identity: |
20203 |
| Gene: |
NOSTRIN |
More about : NOSTRIN |
| Long gene name: |
nitric oxide synthase trafficking |
| Synonyms gene name: |
nitric oxide synthase trafficker |
| Locus: |
2q24, 3 |
| Discovery year: |
2003-01-06 |
| GenBank acession: |
AJ532842 |
| Entrez gene record: |
115677 |
| Pubmed identfication: |
12446846 |
| RefSeq identity: |
NM_052946 |
| Classification: |
F-BAR domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000153990 |