| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
Golgi phosphoprotein 6 |
| Specificity: |
This antibody reacts specifically with GORASP2/Golgi phosphoprotein 6/GRASP55 |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human Golgi phosphoprotein 6 |
| Gene ID: |
26003 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
Both GRASP55 and GRASP65 function in the stacking of Golgi Cisternae (2, ERK2 directly phosphorylates GRASP55, GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi, GRASP55 is highly homologous to GRASP65 and contains two PDZ domains, GRASP55 is myristoylated and palmitoylated, The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, Unlike GRASP65, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure (4), suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis (5), the golgins and other peripheral membrane components such as GRASP55 and GRASP65 (1), which is phosphorylated in mitotic cells, 3), The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
GRASP55 GRS2 GOLPH6 |
| Also known as: |
GORASP2/Golgi phosphoprotein 6/GRASP55 Antibody |
| Other name: |
Anti-GORASP2/Golgi phosphoprotein 6/GRASP55 |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Gene target: |
GORASP2/Golgi phosphoprotein 6/GRASP55 |
| Short name: |
Fluor 594, Anti-GORASP2/Golgi phosphoprotein 6/GRASP55 |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-GORASP2/vesicle packing phosphoprotein 6/GRASP55 |
| Identity: |
17500 |
| Gene: |
GORASP2 |
More about : GORASP2 |
| Long gene name: |
golgi reassembly stacking protein 2 |
| Synonyms gene name: |
55 kDa golgi reassembly stacking protein 2, 55kDa , golgi reassembly stacking protein 2 |
| Locus: |
2q31, 1 |
| Discovery year: |
2002-05-23 |
| Entrez gene record: |
26003 |
| Pubmed identfication: |
10487747 |
| Classification: |
PDZ domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000154072 |