| Long name: |
FITC Conjugated, GORASP2/Golgi phosphoprotein 6/GRASP55 Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Golgi phosphoprotein 6 |
| Specificity: |
This is a highly specific antibody against Golgi phosphoprotein 6 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Golgi phosphoprotein 6 |
| Gene ID number: |
26003 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Both GRASP55 and GRASP65 function in the stacking of Golgi Cisternae (2, ERK2 directly phosphorylates GRASP55, GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi, GRASP55 is highly homologous to GRASP65 and contains two PDZ domains, GRASP55 is myristoylated and palmitoylated, The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, Unlike GRASP65, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure (4), suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis (5), the golgins and other peripheral membrane components such as GRASP55 and GRASP65 (1), which is phosphorylated in mitotic cells, 3), The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
GRASP55 GRS2 GOLPH6 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GORASP2/Golgi phosphoprotein 6/GRASP55 Conjugated |
| Short name: |
FITC Conjugated, GORASP2/Golgi phosphoprotein 6/GRASP55 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, GORASP2/vesicle packing phosphoprotein 6/GRASP55 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
17500 |
| Gene: |
GORASP2 |
More about : GORASP2 |
| Long gene name: |
golgi reassembly stacking protein 2 |
| Synonyms gene name: |
55 kDa golgi reassembly stacking protein 2, 55kDa , golgi reassembly stacking protein 2 |
| Locus: |
2q31, 1 |
| Discovery year: |
2002-05-23 |
| Entrez gene record: |
26003 |
| Pubmed identfication: |
10487747 |
| Classification: |
PDZ domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000154072 |