| Category: |
Conjugated Secondary Antibody |
| Conjugation: |
Biotinylated |
| Host Organism: |
Goat |
| Target antigen: |
Rat IgG |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal antibody |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug/ul |
| Tested applications: |
ELISA, IHC-F, IHC-P, WB |
| Recommended dilutions: |
ELISA(1:500-2000), IHC-F(1:500-2000), IHC-P(1:500-2000), WB(1:500-2000) |
| Cross reactivity: |
Rat |
| Background of the target antigen: |
IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response, The IgG molecule has two separate functions, The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants, is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood, to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen, Immunoglobulin G (IgG) |
| Purification method: |
This antibody has been purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to one year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24° |
| Latin name: |
Rattus norvegicus, Capra aegagrus hircus |
| About: |
2 light chains, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, Rats are used to make rat monoclonal anti mouse antibodies, Rats , The IgG antibody has 2 , There are 2 heavy chains, There are less rat- than mouse clones however, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, goat polyclonal antibodies , mouse monoclonal H&, Immunoglobulin gamma, L chain clones or rabbit, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, antibodies, antigen , binding sites, genes from rodents , have 4 parts, of the genus , serum |
| Gene target: |
(H+L) Conjugated |
| Short name: |
Goat Anti- IgG Antibody (H+L) Conjugated |
| Technique: |
IgGs, antibodies against human proteins, antibodies for, antibody Conjugates, goats, Antibody |
| Host: |
It are very good secondary antibodies that are affinity purified and have a good affinity themselves too, Often KLH is coupled to the antigen so anti KLH can be present in the vial, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection, Goat |
| Isotype: |
IgG |
| Label: |
Biotin |
| Species: |
Goats, Rats, Rat |
| Alternative name: |
biotinilated coupled, caprine antibody to-Rat Immunoglobulin G (Antibody to) (heavy and light chain) |
| Alternative technique: |
antibodies |