| Antigen detectable with this ELISA kit: |
V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) |
| Species reactivity: |
Homo sapiens (Human) |
| Range of detection: |
0, 0, 1, 10ng/mL, 2, 5ng/mL, 25ng/mL, 313-20ng/mL The standard curve concentrations used for the ELISA&rsquo, 313ng/mL, 5ng/mL, 625ng/mL, s were 20ng/mL |
| Sensitivity: |
119ng/mL, The minimum detectable dose of this kit is typically less than 0 |
| Application: |
ELISA |
| Sample types tested for this ELISA kit: |
plasma, tissue homogenates and other biological fluids, Serum |
| Time for performing t hourse test: |
4, 5 hoursours |
| Test principle: |
10nm, After TMB substrate solution is added, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated, Next, Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS), The concentration of V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS) in the samples is then determined by comparing the O, The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm &, The microtiter plate provided in this kit has been pre-coated with an antibody specific to V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color, of the samples to the standard curve, only those wells that contain V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (KRAS), D, The test principle applied in this kit is Sandwich enzyme immunoassay, plusmn |
| Storage and handling: |
Do not freeze any of the components of the ELISA kit, store all of the kit's components regrigerated at tempretaures between 2 and 6 degrees Celcius, Upon receipt |
| Advisory: |
Prior to use, briefly centrifuge these liquid components to collect all of the amount on the bottom of the vial, Due to transportation and handling issues small amounts of the liquid components of the ELISA kit may get trapped onto the seal or walls of the tubes |
| Properties: |
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays, E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays |
| Test: |
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED |
| About: |
Rats , There are less rat- than mouse clones however, Rats are used to make rat monoclonal anti mouse antibodies, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Latin name: |
Rattus norvegicus |
| Gene target: |
V-Ki-Ras2 Kirsten Sarcoma Viral Oncogene Homolog (KRAS) |
| Short name: |
V-Ki-Ras2 Kirsten Sarcoma Viral Oncogene Homolog (KRAS) ELISA kit |
| Technique: |
&hellip, A sign detected by the Colorimetric detection OD optical density absorbance at 450 nm between 0 and 4 with an ELISA reader for 450 nm absortion, Also Enzyme-linked immunoassay, ELISA test kits can be sandwich ELISA, ELISA tests are enzyme linked immunoassays to detect human, ELISAs Enzyme-linked immunosorbent assay 90320007 SNOMED code are used by the medical researcher for detection of human, Genprice Inc, In sandwich ELISAS Enzyme-linked immunosorbent assay 9we use captor and detector antibodies, In single antibody ELISAs the antigen is coated and only a detector antibody is used, In this case the ratio on your graph will be inverse proportional with the antigen present in your sample, MeSH code E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay, MeSH code E05 478 566 350 170 for Enzyme-Linked Immunosorbent Assay or ELISA kits, PROMT code 3841327 Immunoabsorbent assay tests, This is the desigation of other diagnostic analyzers and tests, Traditional competition antigen ELISAs are coated with a captor antibody and a competitive antigen is labelled with the chromogen, competitive have the SNOMED name 61461007 and the PROMT code for ELISA readers is 3841330, mouse, mouse or other proteins in serum, plasma, proteins are supplied in coated 96 well plates to be stored at +4°, the recognized authority tax ID 45-4304622 D-U-N-S number - 078440800, urine or biological fluids, C, ELISA kit |
| Host: |
antigen assay ELISA kit 1 Kit (96 Wells), Rat |
| Species: |
Rats, Rat |
| Alternative name: |
V-Ki-Ras2 Kirsten Rat Sarcoma Viral Oncogene Homolog (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog) Enzyme-linked immunosorbent assay reagent |
| Alternative technique: |
elisas |
| Alternative to gene target: |
C-K-RAS and CFC2 and K-RAS2A and K-RAS2B and K-RAS4A and K-RAS4B and KI-RAS and KRAS1 and KRAS2 and NS and NS3 and RASK2, GABAergic and biological process this GO :0032403 and protein complex binding and molecular function this GO :0035022 and positive regulation of Rac protein signal transduction and biological process this GO :0035176 and social behavior and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0043406 and positive regulation of MAP kinase activity and biological process this GO :0043524 and negative regulation of neuron apoptotic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045596 and negative regulation of cell differentiation and biological process this GO :0048011 and neurotrophin TRK receptor signaling pathway and biological process this GO :0048169 and regulation of long-term neuronal synaptic plasticity and biological process this GO :0050900 and leukocyte migration and biological process this GO :0051000 and positive regulation of nitric-oxide synthase activity and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051146 and striated muscle cell differentiation and biological process this GO :0051384 and response to glucocorticoid and biological process this GO :0051385 and response to mineralocorticoid and biological process this GO :0060441 and epithelial tube branching involved in lung morphogenesis and biological process, KRAS and IDBG-23889 and ENSG00000133703 and 3845, KRAS and IDBG-638783 and ENSBTAG00000009778 and 541140, Kras and IDBG-197719 and ENSMUSG00000030265 and 16653, Plasma membranes, protein complex binding, this GO :0000165 and MAPK cascade and biological process this GO :0000186 and activation of MAPKK activity and biological process this GO :0001934 and positive regulation of protein phosphorylation and biological process this GO :0003924 and GTPase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005525 and GTP binding and molecular function this GO :0005622 and intracellular and cellular component this GO :0005739 and mitochondrion and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006184 and GTP catabolic process and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0006913 and nucleocytoplasmic transport and biological process this GO :0007165 and signal transduction and biological process this GO :0007173 and epidermal growth factor receptor signaling pathway and biological process this GO :0007264 and small GTPase mediated signal transduction and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007411 and axon guidance and biological process this GO :0007596 and blood coagulation and biological process this GO :0008284 and positive regulation of cell proliferation and biological process this GO :0008286 and insulin receptor signaling pathway and biological process this GO :0008542 and visual learning and biological process this GO :0008543 and fibroblast growth factor receptor signaling pathway and biological process this GO :0010628 and positive regulation of gene expression and biological process this GO :0015031 and protein transport and biological process this GO :0016020 and membrane and cellular component this GO :0019002 and GMP binding and molecular function this GO :0019003 and GDP binding and molecular function this GO :0019221 and cytokine-mediated signaling pathway and biological process this GO :0030036 and actin cytoskeleton organization and biological process this GO :0030275 and LRR domain binding and molecular function this GO :0032228 and regulation of synaptic transmission, this GO :0003924 : GTPase activity, this GO :0003924 : GTPase activity and also this GO :0005515 : protein binding and also this GO :0005525 : GTP binding and also this GO :0019002 : GMP binding and also this GO :0019003 : GDP binding and also this GO :0030275 : LRR domain binding and also this GO :0032403 : protein complex binding, this GO :0005515 : protein binding, this GO :0005525 : GTP binding, this GO :0019002 : GMP binding, this GO :0019003 : GDP binding, this GO :0030275 : LRR domain binding, this GO :0032403 : protein complex binding, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Identity: |
6407 |
| Gene: |
KRAS |
More about : KRAS |
| Long gene name: |
GTPase , KRAS proto-oncogene |
| Synonyms gene: |
KRAS2 |
| Synonyms gene name: |
v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog Kirsten rat sarcoma viral oncogene homolog |
| Synonyms: |
KRAS1 |
| Locus: |
12p12, 1 |
| Discovery year: |
1988-06-02 |
| GenBank acession: |
BC010502 |
| Entrez gene record: |
3845 |
| RefSeq identity: |
NM_033360 |
| Classification: |
RAS type GTPase family |
| Havana BLAST/BLAT: |
OTTHUMG00000171193 |
| Locus Specific Databases: |
LRG_344 |