| Type: |
Conjugated Primary Antibody |
| Conjugated with: |
594, ALEXA FLUOR® |
| Host organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Protein/Peptide: |
NCF1/p47 phox |
| Specificity: |
This antibody reacts specifically with NCF1/p47 phox |
| Modification: |
No modification has been applied to this antibody |
| Modification site: |
None |
| Clonality: |
Polyclonal Antibody |
| Clone: |
Polyclonal Antibodies |
| Concentration: |
1ug per 1ul |
| Antigen Source: |
KLH conjugated synthetic peptide derived from human NCF1 |
| Gene ID: |
653361 |
| Swiss Prot: |
P14598 |
| Applications: |
IF(IHC-P) |
| Applications with corresponding dilutions: |
IF(IHC-P)(1:50-200) |
| Cross reactive species: |
Mouse (Mus musculus), Rat (Rattus norvegicus), Human (Homo sapiens) |
| Cross Reactive Species details: |
However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species, No significant cross reactivity has been observed for this antibody for the tested species |
| Background information: |
NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production), NCF2 |
| Purification method: |
Purified by Protein A |
| Storage: |
50% glycerol and 0, Store at 4°, 09% sodium azide, C for 12 months, Water buffered solution containing 100ug/ml BSA |
| Excitation emission: |
590nm/617nm |
| Synonyms: |
p47phox NOXO2 NCF1A SH3PXD1A |
| Also known as: |
NCF1/p47 phox Polyclonal Antibody |
| Other name: |
Anti-NCF1/p47 phox Polyclonal |
| Advisory: |
For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube, Prior to use briefly centrifuge the vial to gather all the solution on the bottom, specificity and sensitivity, thus reducing its reactivity, Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody |
| Properties: |
For facs or microscopy Alexa 1 conjugate |
| Conjugation: |
Alexa Fluor |
| Group: |
Polyclonals and antibodies |
| About: |
The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera, immunohistochemistry on frozen slices or parrafin fixed tissues, Polyclonals can be used for Western blot |
| Gene target: |
NCF1/p47 phox |
| Short name: |
Anti-NCF1/p47 phox (Polyclonal) Fluor 594 |
| Technique: |
Pabs are a collection of immunoglobulin , Polyclonal antibodies , each identifying a different , whereas , (pAbs) are mostly rabbit or goat , B cells, Polyclonal, antibodies , antigen, come from a single N cell lineage, epitope, molecules that react against a specific , monoclonal antibodies , that are secreted by different |
| Label: |
ALEXA |
| Alternative name: |
ALEXA Fluor 594, antibody to-neutrophil cytosolic factor 1/p47 phox (polyclonal) |
| Alternative technique: |
polyclonals |
| Alternative to gene target: |
NCF1 and IDBG-21570 and ENSG00000158517 and 653361, NCF1 and IDBG-639883 and ENSBTAG00000003305 and 281345, Ncf1 and IDBG-202592 and ENSMUSG00000015950 and 17969, Plasma membranes, TAP-dependent and biological process this GO :0002679 and respiratory burst involved in defense response and biological process this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005791 and rough endoplasmic reticulum and cellular component this GO :0005794 and this GO lgi apparatus and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006612 and protein targeting to membrane and biological process this GO :0006691 and leukotriene metabolic process and biological process this GO :0006801 and superoxide metabolic process and biological process this GO :0006915 and apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0006968 and cellular defense response and biological process this GO :0008283 and cell proliferation and biological process this GO :0009055 and electron carrier activity and molecular function this GO :0009617 and response to bacterium and biological process this GO :0016020 and membrane and cellular component this GO :0016175 and superoxide-generating NADPH oxidase activity and molecular function this GO :0017124 and SH3 domain binding and molecular function this GO :0019898 and extrinsic component of membrane and cellular component this GO :0030425 and dendrite and cellular component this GO :0032010 and pha this GO lysosome and cellular component this GO :0035091 and phosphatidylinositol binding and molecular function this GO :0042554 and superoxide anion generation and biological process this GO :0042590 and antigen processing and presentation of exogenous peptide antigen via MHC class I and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0043020 and NADPH oxidase complex and cellular component this GO :0043025 and neuronal cell body and cellular component this GO :0043325 and phosphatidylinositol-3, phosphatidylinositol-3, this GO :0001878 and response to yeast and biological process this GO :0001909 and leukocyte mediated cytotoxicity and biological process this GO :0002474 and antigen processing and presentation of peptide antigen via MHC class I and biological process this GO :0002479 and antigen processing and presentation of exogenous peptide antigen via MHC class I, this GO :0005515 : protein binding, this GO :0005515 : protein binding and also this GO :0009055 : electron carrier activity and also this GO :0016175 : superoxide-generating NADPH oxidase activity and also this GO :0017124 : SH3 domain binding and also this GO :0035091 : phosphatidylinositol binding and also this GO :0043325 : phosphatidylinositol-3, this GO :0009055 : electron carrier activity, this GO :0016175 : superoxide-generating NADPH oxidase activity, this GO :0017124 : SH3 domain binding, this GO :0035091 : phosphatidylinositol binding, this GO :0043325 : phosphatidylinositol-3, 4-bisphosphate binding, 4-bisphosphate binding, 4-bisphosphate binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045730 and respiratory burst and biological process this GO :0045986 and negative regulation of smooth muscle contraction and biological process this GO :0050665 and hydrogen peroxide biosynthetic process and biological process this GO :0050830 and defense response to Gram-positive bacterium and biological process this GO :0050832 and defense response to fungus and biological process this GO :0051701 and interaction with host and biological process this GO :0055114 and oxidation-reduction process and biological process this GO :0070946 and neutrophil mediated killing of gram-positive bacterium and biological process this GO :0070947 and neutrophil mediated killing of fungus and biological process this GO :0090382 and pha this GO some maturation and biological process, neutrophil cytosolic factor 1 |
| Identity: |
7660 |
| Gene: |
NCF1 |
More about : NCF1 |
| Long gene name: |
neutrophil cytosolic factor 1 |
| Synonyms gene name: |
autosomal 1) , chronic granulomatous disease, neutrophil cytosolic factor 1 (47kD |
| Synonyms name: |
NADPH oxidase organizer 2 chronic granulomatous disease, autosomal 1 |
| Locus: |
7q11, 23 |
| Discovery year: |
1989-05-25 |
| GenBank acession: |
M25665 |
| Entrez gene record: |
653361 |
| RefSeq identity: |
NM_000265 |
| Havana BLAST/BLAT: |
OTTHUMG00000149965 |
| Locus Specific Databases: |
NCF1base: Mutation registry for autosomal recessive chronic granulomatous disease (CGD), deficiency of p47phox LRG_87 |