| Also known as: |
CD45 (T200) |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD45 (T200) |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
Rat IgG2a |
| Clone: |
4, YAML 501 |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, ), CD45 (T200) (This antibody is specific for human CD45 |
| Form/Appearance: |
FITC |
| Concentration: |
1 miligram per 1 mililiter |
| Storage and shipping: |
Avoid freeze/thaw cycles, Biotin, DO NOT FREEZE APC and PE conjugates, FITC), For long term storage (Purified, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius, in volumes appropriate for single usage, store the antibody at +4 degrees Celsius, For all formats |
| Tested applications:: |
Western Blot (WB), Flow Cytometry (FC/FACS) |
| Properties: |
Depending on the epitopes used human ELISA kits can be cross reactive to many other species, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, Human proteins, MBS mono FITC is highly sensitive to pH extremes, Mainly analyzed are human serum, Modern , When excited at 488 nanometers, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies, human cell culture supernatants and biological samples, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, plasma, primarily , saliva, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, urine, , (Homo sapiens, Homo sapiens sapiens), This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, humans , ssp |
| Conjugation: |
Anti-FITC Antibody |
| About: |
Rats , There are less rat- than mouse clones however, Rats are used to make rat monoclonal anti mouse antibodies, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Latin name: |
Rattus norvegicus |
| Gene target: |
(CD45 (T200) YAML 501, 4) IgG2a) |
| Short name: |
4) ( IgG2a), Anti- (CD45 (T200)) FITC (clone YAML 501 |
| Technique: |
Fluorescein, FITC |
| Host: |
Rat |
| Isotype: |
IgG2a |
| Label: |
FITC |
| Species: |
Humans, Rats, Rat |
| Alternative name: |
(Rat IgG2a), fluorecein (clonality YAML 501, sapiens (CD45 (T200)), 4), antibody to-H |
| Alternative technique: |
fluorescine |
| Identity: |
9666 |
| Gene: |
PTPRC |
More about : PTPRC |
| Long gene name: |
protein tyrosine phosphatase, receptor type C |
| Synonyms gene: |
CD45 |
| Synonyms: |
LCA T200 GP180 |
| Locus: |
1q31, 1 , 3-q32 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
Y00062 |
| Entrez gene record: |
5788 |
| Pubmed identfication: |
2169617 |
| RefSeq identity: |
NM_002838 |
| Classification: |
Fibronectin type III domain containing CD molecules Protein tyrosine phosphatases, receptor type |
| Havana BLAST/BLAT: |
OTTHUMG00000035702 |
| Locus Specific Databases: |
PTPRCbase: Mutation registry for CD45 deficiency LRG_95 |