| Also known as: |
CD4 |
| Other names: |
CD4 antigen (p55), CD4 molecule, CD4 protein, CD4 receptor, N/A, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, CD4 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD4 |
| Other gene names: |
CD4, CD4 receptor, CD4mut, CD4 |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
Mouse IgG1 |
| Clone: |
W3/25 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Rat (Rattus norvegicus) |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, CD4 (Rat CD4) |
| Form/Appearance: |
(Purified from ascitic fluid via Protein G Chromatography)), 0, 02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml, FITC (FITC conjugated Ig buffered in PBS |
| Concentration: |
1 miligram per 1 mililiter, Antibody Concentration: 0 |
| Storage and shipping: |
Avoid freeze/thaw cycles, Avoid prolonged exposure to light, For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius, in volumes appropriate for single usage, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Immunofluorescence (IF), Immunohistochemistry (IHC) |
| Description: |
GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones, Used offer coupled to magnetic beads, CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| About: |
Rats , There are less rat- than mouse clones however, Rats are used to make rat monoclonal anti mouse antibodies, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Latin name: |
Rattus norvegicus |
| Gene target: |
CD4 W3/25) IgG1) |
| Short name: |
Anti- CD4 FITC (Clone W3/25) (mouse IgG1) |
| Technique: |
Fluorescein, FITC |
| Host: |
Rat |
| Isotype: |
IgG1 |
| Label: |
FITC |
| Species: |
Rats, Rat |
| Alternative name: |
antibody to-Rat CD4 molecule fluorecein (clonality W3/25) (mouse IgG1) |
| Alternative technique: |
fluorescine |
| Alternative to gene target: |
CD4 and IDBG-15034 and ENSG00000010610 and 920, CD4 and IDBG-640247 and ENSBTAG00000003255 and 407098, CD4mut, Cd4 and IDBG-188351 and ENSMUSG00000023274 and 12504, Cell surfaces, protein homodimerization activity, this GO :0001816 and cytokine production and biological process this GO :0001948 and glycoprotein binding and molecular function this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005201 and extracellular matrix structural constituent and molecular function this GO :0005515 and protein binding and molecular function this GO :0005769 and early endosome and cellular component this GO :0005788 and endoplasmic reticulum lumen and cellular component this GO :0005789 and endoplasmic reticulum membrane and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006948 and induction by virus of host cell-cell fusion and biological process this GO :0006955 and immune response and biological process this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007167 and enzyme linked receptor protein signaling pathway and biological process this GO :0007169 and transmembrane receptor protein tyrosine kinase signaling pathway and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0015026 and coreceptor activity and molecular function this GO :0016021 and integral component of membrane and cellular component this GO :0016032 and viral process and biological process this GO :0019899 and enzyme binding and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0030217 and T cell differentiation and biological process this GO :0030260 and entry into host cell and biological process this GO :0031295 and T cell costimulation and biological process this GO :0032507 and maintenance of protein location in cell and biological process this GO :0042101 and T cell receptor complex and cellular component this GO :0042110 and T cell activation and biological process this GO :0042289 and MHC class II protein binding and molecular function this GO :0042803 and protein homodimerization activity and molecular function this GO :0045058 and T cell selection and biological process this GO :0045086 and positive regulation of interleukin-2 biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045234 and protein palmitoleylation and biological process this GO :0045860 and positive regulation of protein kinase activity and biological process this GO :0050690 and regulation of defense response to virus by virus and biological process this GO :0050731 and positive regulation of peptidyl-tyrosine phosphorylation and biological process this GO :0050829 and defense response to Gram-negative bacterium and biological process this GO :0050850 and positive regulation of calcium-mediated signaling and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0050863 and regulation of T cell activation and biological process this GO :0050870 and positive regulation of T cell activation and biological process, this GO :0001948 : glycoprotein binding, this GO :0001948 : glycoprotein binding and also this GO :0004872 : receptor activity and also this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005201 : extracellular matrix structural constituent and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0015026 : coreceptor activity and also this GO :0019899 : enzyme binding and also this GO :0019901 : protein kinase binding and also this GO :0042289 : MHC class II protein binding and also this GO :0042803 : protein homodimerization activity, this GO :0004872 : receptor activity, this GO :0004888 : transmembrane signaling receptor activity, this GO :0005201 : extracellular matrix structural constituent, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0015026 : coreceptor activity, this GO :0019899 : enzyme binding, this GO :0019901 : protein kinase binding, this GO :0042289 : MHC class II protein binding, this GO :0042803 : protein homodimerization activity, CD4 molecule |
| Identity: |
1678 |
| Gene: |
CD4 |
More about : CD4 |
| Long gene name: |
CD4 molecule |
| Synonyms gene name: |
CD4 antigen (p55) T-cell surface glycoprotein CD4 |
| Locus: |
12p13, 31 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
M35160 |
| Entrez gene record: |
920 |
| RefSeq identity: |
NM_000616 |
| Classification: |
C2-set domain containing CD molecules V-set domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000168514 |