| Category: |
Conjugated Secondary Antibody |
| Conjugation: |
Biotinylated |
| Host Organism: |
Goat |
| Target antigen: |
Mouse IgM |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal antibody |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug/ul |
| Tested applications: |
ELISA, IHC-F, IHC-P, WB |
| Recommended dilutions: |
ELISA(1:500-2000), IHC-F(1:500-2000), IHC-P(1:500-2000), WB(1:500-2000) |
| Cross reactivity: |
Mouse |
| Background of the target antigen: |
IgM antibody is prominent in early immune responses to most antigens and is largely confined to plasma due to it's large size, Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells, Immunoglobulin IgM normally constitutes about 10% of serum immunoglobulins |
| Purification method: |
This antibody has been purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to one year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, IgM is by far the physically largest antibody in the human circulatory system, Immunoglobulin M, It is the first antibody to appear in response to initial exposure to an antigen, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, is a basic , or , 6, B cells, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Secondary Antibodies they should be stored frozen at - 24°, IgM , antibody , for short, that is produced by |
| Latin name: |
Mus musculus, Capra aegagrus hircus |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Gene target: |
(H+L) Conjugated |
| Short name: |
Goat Anti-Mouse IgM Antibody (H+L) Conjugated |
| Technique: |
IgMs, antibodies against human proteins, antibodies for, antibody Conjugates, goats, mouses, Antibody |
| Host: |
It are very good secondary antibodies that are affinity purified and have a good affinity themselves too, Often KLH is coupled to the antigen so anti KLH can be present in the vial, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection, Goat |
| Isotype: |
IgM |
| Label: |
Biotin |
| Species: |
Goats, Mouses, Mouse |
| Alternative name: |
biotinilated coupled, caprine antibody to-Mouse Immunoglobulin M (Antibody to) (heavy and light chain) |
| Alternative technique: |
antibodies |