| Alternative name 1: |
Cont, FITC + Ms IgG1 (Clone:DAK-G01), Neg, RPE, RPE-Cy5 + Mouse IgG1 (Clone:DAK-G01), tri-label, CD45 (Clone:T29/33) |
| Alternative name 2: |
Cont, FITC + Ms IgG1 (Clone:DAK-G01), Neg, RPE, RPE-Cy5 + Mouse IgG1 (Clone:DAK-G01), tri-label, CD45 (Clone:T29/33) |
| Alternative name 3: |
Cont, FITC + Ms IgG1 (Clone:DAK-G01), Neg, RPE, RPE-Cy5 + Mus musculus IgG1 (Clone:DAK-G01), tri-label, CD45 (Clone:T29/33) |
| Alternative name 4: |
Cont, tri-label, anti-CD45 Clone:T29/33 + IgG1 Clone:DAK-G01 + Ms IgG1 Clone:DAK-G01 Neg |
| Alternative name 5: |
Cont, tri-label, CD45 Clone:T29/33 + IgG1 Clone:DAK-G01 + Ms IgG1 Clone:DAK-G01 Neg |
| Clonality: |
Monoclonal Antibody |
| Description: |
Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, accurate-monoclonals FITC is highly sensitive to pH extremes, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor |
| Conjugation: |
Anti-FITC Antibody, 5, Cy5 |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
Cont, Neg, tri, CD45 :T29/33) RPE + IgG1 :DAK-G01) + Ms IgG1 :DAK-G01) RPE |
| Short name: |
Cont, Neg, tri-label, CD45 (Clone:T29/33) RPE- + Mouse IgG1 (Clone:DAK-G01) FITC + Ms IgG1 (Clone:DAK-G01) RPE |
| Technique: |
Fluorescein, mouses, Mouse |
| Isotype: |
IgG1 |
| Label: |
Cy5 |
| Species: |
Mouses, Mouse |
| Alternative name: |
Cont, Neg, RPE, RPE-cyanine 5 and Mouse IgG1 (clonality:DAK-G01), fluorecein and Ms IgG1 (clonality:DAK-G01), tri-label, CD45 (clonality:T29/33) |
| Alternative technique: |
murine |
| Identity: |
9666 |
| Gene: |
PTPRC |
More about : PTPRC |
| Long gene name: |
protein tyrosine phosphatase, receptor type C |
| Synonyms gene: |
CD45 |
| Synonyms: |
LCA T200 GP180 |
| Locus: |
1q31, 1 , 3-q32 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
Y00062 |
| Entrez gene record: |
5788 |
| Pubmed identfication: |
2169617 |
| RefSeq identity: |
NM_002838 |
| Classification: |
Fibronectin type III domain containing CD molecules Protein tyrosine phosphatases, receptor type |
| Havana BLAST/BLAT: |
OTTHUMG00000035702 |
| Locus Specific Databases: |
PTPRCbase: Mutation registry for CD45 deficiency LRG_95 |