| Alternative name 1: |
Clone: MEC14, FITC conj, Rat Mab anti-Mouse, 7, CD34 |
| Alternative name 2: |
Clone: MEC14, FITC conj, Rat Mab antibody-Mouse, 7, CD34 |
| Alternative name 3: |
Clone: MEC14, FITC conj, Rattus norvegicus Mab antibody-Mus musculus, 7, CD34 |
| Alternative name 4: |
7, anti-CD34 Clone: MEC14 |
| Alternative name 5: |
7, CD34 Clone: MEC14 |
| Clonality: |
Monoclonal Antibody |
| Description: |
Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, The most used affinity clone is QBEND10, This accurate-monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, We compare the affinity to this clone, When excited at 488 nanometers, accurate-monoclonals FITC is highly sensitive to pH extremes, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, Stem cell or hematopoietic marker |
| Conjugation: |
Anti-FITC Antibody |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Rats are used to make rat monoclonal anti mouse antibodies, Rats , There are less rat- than mouse clones however, Monoclonals of this antigen are available in different clones, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Rattus norvegicus, Mus musculus |
| French translation: |
anticorps |
| Gene target: |
Monoclonal MEC14, conj, 7, CD34 |
| Short name: |
Clone: MEC14, FITC conj, Mouse Monoclonal antibody-Mouse, 7, CD34 |
| Technique: |
Fluorescein, Monoclonals or monoclonal antibodies, mouses, Mouse |
| Host: |
Rat |
| Label: |
FITC |
| Species: |
Mouses, Rats, Mouse |
| Alternative name: |
Rat Mouse monoclonal (Antibody to)-Mouse, clonality: MEC14, fluorecein conj, 7, CD34 molecule |
| Alternative technique: |
murine |
| Alternative to gene target: |
CD34 and IDBG-106399 and ENSG00000174059 and 947, CD34 and IDBG-638449 and ENSBTAG00000010562 and 281051, Cd34 and IDBG-209317 and ENSMUSG00000016494 and 12490, Extracellular, sulfate binding, this GO :0001894 and tissue homeostasis and biological process this GO :0001935 and endothelial cell proliferation and biological process this GO :0003094 and glomerular filtration and biological process this GO :0003158 and endothelium development and biological process this GO :0005576 and extracellular region and cellular component this GO :0005737 and cytoplasm and cellular component this GO :0005764 and lysosome and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006536 and glutamate metabolic process and biological process this GO :0007155 and cell adhesion and biological process this GO :0007165 and signal transduction and biological process this GO :0008134 and transcription factor binding and molecular function this GO :0008217 and regulation of blood pressure and biological process this GO :0008283 and cell proliferation and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0009925 and basal plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0010628 and positive regulation of gene expression and biological process this GO :0010629 and negative regulation of gene expression and biological process this GO :0016020 and membrane and cellular component this GO :0016324 and apical plasma membrane and cellular component this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0030097 and hemopoiesis and biological process this GO :0030195 and negative regulation of blood coagulation and biological process this GO :0030246 and carbohydrate binding and molecular function this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0032733 and positive regulation of interleukin-10 production and biological process this GO :0035759 and mesangial cell-matrix adhesion and biological process this GO :0036053 and glomerular endothelium fenestra and cellular component this GO :0038001 and paracrine signaling and biological process this GO :0042482 and positive regulation of odontogenesis and biological process this GO :0043199 and sulfate binding and molecular function this GO :0045019 and negative regulation of nitric oxide biosynthetic process and biological process this GO :0045171 and intercellular bridge and cellular component this GO :0045766 and positive regulation of angiogenesis and biological process this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0048870 and cell motility and biological process this GO :0050776 and regulation of immune response and biological process this GO :0050900 and leukocyte migration and biological process this GO :0060290 and transdifferentiation and biological process this GO :0061042 and vascular wound healing and biological process this GO :0071425 and hematopoietic stem cell proliferation and biological process this GO :0071636 and positive regulation of transforming growth factor beta production and biological process this GO :0071657 and positive regulation of granulocyte colony-stimulating factor production and biological process this GO :0071944 and cell periphery and cellular component this GO :0071971 and extracellular vesicular exosome assembly and biological process this GO :0072011 and glomerular endothelium development and biological process this GO :0072089 and stem cell proliferation and biological process this GO :0072254 and metanephric glomerular mesangial cell differentiation and biological process this GO :1900035 and negative regulation of cellular response to heat and biological process this GO :1900038 and negative regulation of cellular response to hypoxia and biological process this GO :1900041 and negative regulation of interleukin-2 secretion and biological process this GO :1900168 and positive regulation of glial cell line-derived neurotrophic factor secretion and biological process this GO :1901215 and negative regulation of neuron death and biological process this GO :2001214 and positive regulation of vasculogenesis and biological process, this GO :0008134 : transcription factor binding, this GO :0008134 : transcription factor binding and also this GO :0030246 : carbohydrate binding and also this GO :0043199 : sulfate binding, this GO :0030246 : carbohydrate binding, this GO :0043199 : sulfate binding, CD34 molecule |
| Identity: |
1662 |
| Gene: |
CD34 |
More about : CD34 |
| Long gene name: |
CD34 molecule |
| Synonyms gene name: |
CD34 antigen |
| Locus: |
1q32, 2 |
| Discovery year: |
1990-09-10 |
| GenBank acession: |
M81104 |
| Entrez gene record: |
947 |
| Pubmed identfication: |
1370171 1374051 |
| RefSeq identity: |
NM_001773 |
| Classification: |
CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000036565 |