| Alternative name 1: |
Clone: 3A4, Mab anti-, RFFL |
| Alternative name 2: |
Clone: 3A4, Mab antibody-, RFFL |
| Alternative name 3: |
Clone: 3A4, Mab antibody-, RFFL |
| Alternative name 4: |
anti-RFFL Clone: 3A4 |
| Alternative name 5: |
RFFL Clone: 3A4 |
| Clonality: |
Monoclonal Antibody |
| Description: |
Amongst the advantages of the monoclonal antibodies are: the fact that while the hybridoma takes a bit longer to be produced, If used correctly and according the protocol, in comparison to the polyclonal antibodies monoclonal antibodies are highly specific to a given epitope and can be used in applications where specific targeting is required, once the line is ready there is virtually an endless supply of these antibodie throughout time with little to no variations in recognition sites of the antigen in different batches, this antibody will provide excellent and reproducible results with guaranteed success for the tested and confirmed applications, This is a monoclonal antibody which is greatly purified and with high binding affinity for the antigen that it is risen against |
| Storage and handling: |
Antibodies in a liquid form can be shipped and stored for a short period of time at +4 degrees Celsius, antibodies that are lyophilized can be transported at ambient temperature and stored for short terms at +4 degrees Celsius, for long term storage (up to one year) 25-50% glycerol or ethylene glycol should be added and then the container has to be stored at -20°, for longer periods - at -20 , C, Generally |
| Advisory: |
For antibodies in a liquid form or reconstituted lyophilized antibodies small amounts of the soultion could be captured on the cap or the walls of the container, Right before use you could briefly centrifuge the vial to collect all of the solution on the bottom, To keep the quality and the affinity of the antibody cycles of freezing and thawing should be avoided |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Monoclonals of this antigen are available in different clones |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| French translation: |
anticorps |
| Gene target: |
Monoclonal 3A4, RFFL |
| Short name: |
Mouse Monoclonal antibody- Clone: 3A4, RFFL |
| Technique: |
Monoclonals or monoclonal antibodies, mouses, Mouse |
| Species: |
Mouses, Mouse |
| Alternative name: |
Mouse monoclonal (Antibody to)-, clonality: 3A4, ring finger and FYVE-like domain containing E3 ubiquitin protein ligase |
| Alternative technique: |
murine |
| Alternative to gene target: |
Plasma membranes, RFFL and IDBG-41038 and ENSG00000092871 and 117584, RFFL and IDBG-630885 and ENSBTAG00000013645 and 530263, Rffl and IDBG-204769 and ENSMUSG00000020696 and 67338, this GO :0002020 and protease binding and molecular function this GO :0002039 and p53 binding and molecular function this GO :0002947 and tumor necrosis factor receptor superfamily complex and cellular component this GO :0004842 and ubiquitin-protein transferase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005829 and cytosol and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006511 and ubiquitin-dependent protein catabolic process and biological process this GO :0006886 and intracellular protein transport and biological process this GO :0008270 and zinc ion binding and molecular function this GO :0010008 and endosome membrane and cellular component this GO :0010762 and regulation of fibroblast migration and biological process this GO :0010804 and negative regulation of tumor necrosis factor-mediated signaling pathway and biological process this GO :0016020 and membrane and cellular component this GO :0016023 and cytoplasmic membrane-bounded vesicle and cellular component this GO :0016874 and ligase activity and molecular function this GO :0019901 and protein kinase binding and molecular function this GO :0031625 and ubiquitin protein ligase binding and molecular function this GO :0032006 and regulation of TOR signaling and biological process this GO :0043161 and proteasome-mediated ubiquitin-dependent protein catabolic process and biological process this GO :0055038 and recycling endosome membrane and cellular component this GO :0070936 and protein K48-linked ubiquitination and biological process this GO :1901797 and negative regulation of signal transduction by p53 class mediator and biological process this GO :1902042 and negative regulation of extrinsic apoptotic signaling pathway via death domain receptors and biological process this GO :2001271 and negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis and biological process, this GO :0002020 : protease binding, this GO :0002020 : protease binding and also this GO :0002039 : p53 binding and also this GO :0004842 : ubiquitin-protein transferase activity and also this GO :0005515 : protein binding and also this GO :0008270 : zinc ion binding and also this GO :0016874 : ligase activity and also this GO :0019901 : protein kinase binding and also this GO :0031625 : ubiquitin protein ligase binding, this GO :0002039 : p53 binding, this GO :0004842 : ubiquitin-protein transferase activity, this GO :0005515 : protein binding, this GO :0008270 : zinc ion binding, this GO :0016874 : ligase activity, this GO :0019901 : protein kinase binding, this GO :0031625 : ubiquitin protein ligase binding, ubiquitin protein ligase binding, ring finger and FYVE-like domain containing E3 ubiquitin protein ligase |
| Identity: |
24821 |
| Gene: |
RFFL |
More about : RFFL |
| Long gene name: |
ring finger and FYVE like domain containing E3 ubiquitin protein ligase |
| Synonyms gene name: |
ring finger and FYVE-like domain containing |
| Synonyms: |
rififylin fring RNF189 RNF34L CARP2 CARP-2 |
| Synonyms name: |
caspase 8 and 10 associated RING protein-2 |
| Locus: |
17q12 |
| Discovery year: |
2005-08-16 |
| GenBank acession: |
AF434816 |
| Entrez gene record: |
117584 |
| Pubmed identfication: |
15229288 |
| RefSeq identity: |
NM_057178 |
| Classification: |
Ring finger proteins |
| Havana BLAST/BLAT: |
OTTHUMG00000132933 |