| Also known as: |
CD14 |
| Other names: |
CD14 antigen, CD14 molecule<, CD_antigen: CD14, Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, CD14 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD14 |
| Other gene names: |
CD14, N/A, N/A, CD14 |
| Clonality: |
Monoclonal antibody |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
MEM18 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold, For this purpose, In addition and to control lot-to-lot variation, In practice, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold, The sensitivity of MEM18 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity), This particular antibody might cross react with speacies outside of the listed ones, a mAb-concentration range is selected to include both the saturation point (i, the given lot is compared and adjusted to fluorescence standards with defined intensity, the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i, the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells), The CD14 mAb (clone MEM18) recognizes surface CD14 on human monocytes and macrophages as well as on neutrophils, e, e |
| Form/Appearance: |
0, 1% BSA, 05% NaN3, 2, PBS pH 7 |
| Storage and shipping: |
Keep the antibody at +4 degrees Celsius and do NOT freeze it, Please refer to the expiry date printed on the vial, The use of the reagent after the expiration date is not recommended, To enchance the antibody's stability sodium azide has been added, the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light, the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody |
| Tested applications:: |
Flow Cytometry (FC/FACS), Indirect Immunofluorescence (IF), Direct Immunofluorescence (IF) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
conjugated, CD14 |
| Short name: |
conjugated FITC Antibody, Mouse Anti-CD14 |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, mouses, Antibody |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
coupled to fluorecein (Antibody to), Mouse antibody to-CD14 molecule |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
CD14 and IDBG-49157 and ENSG00000170458 and 929, CD14 and IDBG-646064 and ENSBTAG00000015032 and 281048, Cd14 and IDBG-138525 and ENSMUSG00000051439 and 12475, Extracellular, lipoteichoic acid binding, this GO :0001530 and lipopolysaccharide binding and molecular function this GO :0001847 and opsonin receptor activity and molecular function this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002237 and response to molecule of bacterial origin and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0006909 and pha this GO cytosis and biological process this GO :0006915 and apoptotic process and biological process this GO :0006954 and inflammatory response and biological process this GO :0007166 and cell surface receptor signaling pathway and biological process this GO :0007249 and I-kappaB kinase/NF-kappaB signaling and biological process this GO :0009408 and response to heat and biological process this GO :0009986 and cell surface and cellular component this GO :0010008 and endosome membrane and cellular component this GO :0016019 and peptidoglycan receptor activity and molecular function this GO :0031225 and anchored component of membrane and cellular component this GO :0032026 and response to magnesium ion and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032760 and positive regulation of tumor necrosis factor production and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0034612 and response to tumor necrosis factor and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0045471 and response to ethanol and biological process this GO :0045807 and positive regulation of endocytosis and biological process this GO :0050715 and positive regulation of cytokine secretion and biological process this GO :0051602 and response to electrical stimulus and biological process this GO :0070062 and extracellular vesicular exosome and cellular component this GO :0070891 and lipoteichoic acid binding and molecular function this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071223 and cellular response to lipoteichoic acid and biological process, this GO :0001530 : lipopolysaccharide binding, this GO :0001530 : lipopolysaccharide binding and also this GO :0001847 : opsonin receptor activity and also this GO :0005515 : protein binding and also this GO :0016019 : peptidoglycan receptor activity and also this GO :0070891 : lipoteichoic acid binding, this GO :0001847 : opsonin receptor activity, this GO :0005515 : protein binding, this GO :0016019 : peptidoglycan receptor activity, this GO :0070891 : lipoteichoic acid binding, CD14 molecule |
| Identity: |
1628 |
| Gene: |
CD14 |
More about : CD14 |
| Long gene name: |
CD14 molecule |
| Synonyms gene name: |
CD14 antigen |
| Locus: |
5q31, 3 |
| Discovery year: |
1988-05-31 |
| Entrez gene record: |
929 |
| Pubmed identfication: |
2472171 2462937 |
| RefSeq identity: |
NM_000591 |
| Classification: |
CD molecules Scavenger receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000129507 |