| Also known as: |
CD65s |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD65s |
| Clonality: |
Monoclonal antibody |
| Immunoglobulin isotype: |
IgM |
| Clone: |
VIM2 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
50ul of leukocytes containing 10^7 cells/ml are stained with 20ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold, For this purpose, In addition and to control lot-to-lot variation, In practice, Similar results were obtained by Kniep et al, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The epitope recognized was shown by Macher et al, The final concentration of the product is then adjusted to be at least three-fold above the detection threshold, The sensitivity of VIM2 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescenceintensity), This particular antibody might cross react with speacies outside of the listed ones, Together with other sialylated and fucosylated polylactosamines the carbohydrate structure recognized by VIM2 may play a critical role on the adhesion of granulocytes and monocytes to endothelium and platelets during inflammation and clotting, a mAb-concentration range is selected to include both the saturation point (i, the given lot is compared and adjusted to fluorescence standards with defined intensity, the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i, the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells), to involve a defined sialofucooligosaccharide sequence, Antibody VIM2 reacts with a carbohydrate structure expressed by myeloid cells, e, e |
| Form/Appearance: |
0, 1% BSA, 05% NaN3, 2, PBS pH 7 |
| Storage and shipping: |
Keep the antibody at +4 degrees Celsius and do NOT freeze it, Please refer to the expiry date printed on the vial, The use of the reagent after the expiration date is not recommended, To enchance the antibody's stability sodium azide has been added, the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light, the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody |
| Tested applications:: |
Flow Cytometry (FC/FACS), Indirect Immunofluorescence (IF), Direct Immunofluorescence (IF) |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
CD65s |
| Short name: |
Mouse Anti-CD65s |
| Technique: |
mouses, Mouse |
| Species: |
Mouses, Mouse |
| Alternative name: |
Mouse antibody to-CD65s |
| Alternative technique: |
murine |