| Also known as: |
CD45 |
| Other names: |
C, CD45 antigen, CD_antigen: CD45, L-CA, Leukocyte common antigen, Receptor-type tyrosine-protein phosphatase C, T200, T200 glycoprotein, T200 leukocyte common antigen, c polypeptide, partial, protein tyrosine phosphatase, protein tyrosine phosphatase, receptor type, receptor type, receptor-type tyrosine-protein phosphatase C, CD45 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD45 |
| Other gene names: |
B220, CD45, CD45, CD45R, GP180, L-CA, L-CA, LCA, LY5, PTPRC, T200, PTPRC |
| Clonality: |
Monoclonal antibody |
| Immunoglobulin isotype: |
IgG2a |
| Clone: |
VIT200 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold, For this purpose, In addition and to control lot-to-lot variation, In practice, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold, The sensitivity of VIT200 mAb is determined by staining well-definted blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity), This particular antibody might cross react with speacies outside of the listed ones, a mAb-concentration range is selected to include both the saturation point (i, the given lot is compared and adjusted to fluorescence standards with defined intensity, the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i, the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells), The CD45 mAb (clone VIT200) recognizes a pan CD45 epitope, e, e |
| Form/Appearance: |
0, 1% BSA, 05% NaN3, 2, PBS pH 7 |
| Storage and shipping: |
Keep the antibody at +4 degrees Celsius and do NOT freeze it, Please refer to the expiry date printed on the vial, The use of the reagent after the expiration date is not recommended, To enchance the antibody's stability sodium azide has been added, the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light, the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody |
| Tested applications:: |
Flow Cytometry (FC/FACS), Indirect Immunofluorescence (IF), Direct Immunofluorescence (IF) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
conjugated, CD45 |
| Short name: |
conjugated FITC, Mouse Anti-CD45 |
| Technique: |
Fluorescein, mouses, Mouse |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
coupled to fluorecein, Mouse antibody to-CD45 |
| Alternative technique: |
murine |
| Identity: |
9666 |
| Gene: |
PTPRC |
More about : PTPRC |
| Long gene name: |
protein tyrosine phosphatase, receptor type C |
| Synonyms gene: |
CD45 |
| Synonyms: |
LCA T200 GP180 |
| Locus: |
1q31, 1 , 3-q32 |
| Discovery year: |
1986-01-01 |
| GenBank acession: |
Y00062 |
| Entrez gene record: |
5788 |
| Pubmed identfication: |
2169617 |
| RefSeq identity: |
NM_002838 |
| Classification: |
Fibronectin type III domain containing CD molecules Protein tyrosine phosphatases, receptor type |
| Havana BLAST/BLAT: |
OTTHUMG00000035702 |
| Locus Specific Databases: |
PTPRCbase: Mutation registry for CD45 deficiency LRG_95 |