| Also known as: |
Myeloperoxidase-C2 (FITC) and CD22 (PE) |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal antibody |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
RFB4, 8E6 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold, For this purpose, In addition and to control lot-to-lot variation, In practice, In this COMBI-IC Reagent antibody 8E6 is conjugated to FITC, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The CD22 mAb (clone RFB4) recognizes cytoplasmatic CD22 in precursor B-cells and surface as well cytoplasmatic CD22 on mature B-cells, The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold, The sensitivity of MPO-C2/CD22 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity), This particular antibody might cross react with speacies outside of the listed ones, a mAb-concentration range is selected to include both the saturation point (i, antibody RFB4 is conjugated to Phycoeythrin (PE), the given lot is compared and adjusted to fluorescence standards with defined intensity, the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i, the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells), Antibody MPO-C2 (clone 8E2) reacts with human myeloperoxidase (MPO) expressed by normal and malignant myelomonocytic cells, e, e |
| Form/Appearance: |
0, 1 mg/ml BSA, 1% NaN3, 2, PBS pH 7 |
| Storage and shipping: |
Keep the antibody at +4 degrees Celsius and do NOT freeze it, Please refer to the expiry date printed on the vial, The use of the reagent after the expiration date is not recommended, To enchance the antibody's stability sodium azide has been added, the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light, the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody |
| Tested applications:: |
Flow Cytometry (FC/FACS) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| Conjugation: |
Anti-FITC Antibody |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
COMBI IC Reagent: Myeloperoxidase-C2 CD22 (PE) |
| Short name: |
COMBI IC Reagent: Mouse Anti-Myeloperoxidase-C2 (FITC) Mouse Anti-CD22 (PE) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, mouses, Antibody |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
COMBI IC Reagent: Mouse antibody to-Myeloperoxidase-complement component 2 (fluorecein) and Mouse antibody to-CD22 molecule (peroxidase) (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ARMD14 and CO2, C2 and IDBG-124824 and ENSG00000166278 and 101928623, C2 and IDBG-174547 and ENSMUSG00000024371 and 12263, C2 and IDBG-633680 and ENSBTAG00000007450 and 515440, CD22 and IDBG-44001 and ENSG00000012124 and 933, CD22 and IDBG-635610 and ENSBTAG00000011887 and 514582, Cd22 and IDBG-171574 and ENSMUSG00000030577 and 12483, Cell surfaces, Extracellular, SIGLEC-2 and SIGLEC2, classical pathway and biological process this GO :0007584 and response to nutrient and biological process this GO :0030449 and regulation of complement activation and biological process this GO :0045087 and innate immune response and biological process this GO :2000427 and positive regulation of apoptotic cell clearance and biological process, complement component 2, protein binding, protein binding, this GO :0003824 and catalytic activity and molecular function this GO :0004252 and serine-type endopeptidase activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005576 and extracellular region and cellular component this GO :0005615 and extracellular space and cellular component this GO :0006508 and proteolysis and biological process this GO :0006956 and complement activation and biological process this GO :0006958 and complement activation, this GO :0003824 : catalytic activity, this GO :0003824 : catalytic activity and also this GO :0004252 : serine-type endopeptidase activity and also this GO :0005515 : protein binding, this GO :0004252 : serine-type endopeptidase activity, this GO :0005515 and protein binding and molecular function this GO :0005887 and integral component of plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0045087 and innate immune response and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0005515 : protein binding, this GO :0005515 : protein binding, this GO :0005515 : protein binding, 717, CD22 molecule |
| Identity: |
1643 |
| Gene: |
CD22 |
More about : CD22 |
| Long gene name: |
CD22 molecule |
| Synonyms gene name: |
CD22 antigen |
| Synonyms: |
SIGLEC-2 SIGLEC2 |
| Synonyms name: |
sialic acid binding Ig-like lectin 2 |
| Locus: |
19q13, 12 |
| Discovery year: |
1991-06-06 |
| GenBank acession: |
X52785 |
| Entrez gene record: |
933 |
| Pubmed identfication: |
8496602 1691828 |
| RefSeq identity: |
NM_001771 |
| Classification: |
C2-set domain containing CD molecules Sialic acid binding Ig like lectins |
| Havana BLAST/BLAT: |
OTTHUMG00000183298 |