| Also known as: |
Mouse anti FITC |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG1 |
| Clone: |
NI 239 |
| Host organism: |
Mouse (Mus musculus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, N/A |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, Biotin-conjugated purified monoclonal Mouse antibody to fluorescein isothiocyanate isomer 1 |
| Storage and shipping: |
Temperature variations in the range between +1C to +7C are tolerable, Keep the antibody refrigerated at +4 degrees Celsius |
| Tested applications:: |
Dot Blot (DB), Immunoblotting (IB), Immunocytochemistry (IHC), Immunohistochemistry (IHC), ELISA (EIA) |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, When excited at 488 nanometers, alkaline phosphatase substrates or anti biotin conjugated antibodies, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, 6, Biotin conjugates can be detected by horseradish peroxidase |
| Conjugation: |
Anti-FITC Antibody, Biotinylated |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
conjugated with |
| Short name: |
conjugated with, Mouse Anti-FITC |
| Technique: |
Fluorescein, mouses, Mouse |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
coupled including biotinilated, Mouse antibody to-fluorecein |
| Alternative technique: |
murine |