| Also known as: |
CD3e |
| Other names: |
N/A, epsilon (CD3-TCR complex), CD3e molecule |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
CD3e |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
Hamster IgG |
| Clone: |
145-2C11 |
| Host organism: |
Hamster |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby, Mouse (Mus musculus) |
| Specificity and cross-reactivity: |
Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, This particular antibody might cross react with speacies outside of the listed ones, CD3e (Mouse CD3epsilon) |
| Form/Appearance: |
FITC |
| Concentration: |
1 miligram per 1 mililiter, Antibody Concentration: 0 |
| Storage and shipping: |
Avoid prolonged exposure to light, Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity, For long term storage, Keep the unused portions of the antibody in volumes suitable for single use, aliquot and freeze the antibody at -20 degrees Celsius, Store the antibody at +4 degrees Celsius |
| Tested applications:: |
Immunohistochemistry (IHC) |
| Properties: |
FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, MBS mono FITC is highly sensitive to pH extremes, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, This MBS mono Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS |
| Conjugation: |
Anti-FITC Antibody |
| About: |
2 light chains, For storage sodium azide is added or you can call us to request azide free antibody preparations, IgG, The IgG antibody has 2 , There are 2 heavy chains, These will need colder storage temperatures, They represent 70% or more of , This antibody can be antigen purified or protein A or G purified, goat polyclonal antibodies , mouse monoclonal H&, Immunoglobulin gamma, L chain clones or rabbit, antibodies, antigen , binding sites, have 4 parts, serum |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Mus musculus |
| Gene target: |
145-2C11), CD3e |
| Short name: |
FITC (Clone 145-2C11) (hamster IgG), Anti-Mouse CD3e |
| Technique: |
Fluorescein, IgGs, mouses, Mouse |
| Isotype: |
IgG |
| Label: |
FITC |
| Species: |
Mouses, Mouse |
| Alternative name: |
epsilon (CD3-TCR complex), fluorecein (clonality 145-2C11) (hamster Immunoglobulin G), antibody to-Mouse CD3e molecule |
| Alternative technique: |
murine |