| Also known as: |
ITGAM / CD11b |
| Other names: |
CD11 antigen-like family member B, CD11 antigen-like family member B, CD_antigen: CD11b, CR-3 alpha chain, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Integrin alpha-M, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, alpha M (complement component 3 receptor 3 subunit), antigen CD11b (p170), cell surface glycoprotein MAC-1 subunit alpha, integrin, integrin alpha-M, leukocyte adhesion receptor MO1, macrophage antigen alpha polypeptide, neutrophil adherence receptor alpha-M subunit, integrin alpha-M isoform 2 |
| Category: |
Antibodies |
| Subcategory: |
Mnoclonal antibodies |
| Gene name: |
ITGAM |
| Gene name synonims: |
CD11b |
| Other gene names: |
CD11B, CD11B, CR3A, CR3A, ITGAM, MAC-1, MAC1A, MO1A, SLEB6, ITGAM |
| Clonality: |
Monoclonal |
| Immunoglobulin isotype: |
IgG2b |
| Clone: |
15, M1/70 |
| Host organism: |
Rat (Rattus norvegicus) |
| Species reactivity: |
Due to limited knowledge and inability for testing each and every species, Mouse (Mus musculus), Rabbit (Oryctolagus cuniculus), the reactivity of the antibody may extend to other species which are not listed hereby, Human (Homo sapiens) |
| Specificity and cross-reactivity: |
Clone M1/70, NK cells and tissue macrophages, Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages, Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited, The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages, This particular antibody might cross react with speacies outside of the listed ones, This product is routinely tested in flow cytometry on mouse peritoneal macrophages, a differentiation antigen expressed by granulocytes, monocytes, 15 has been reported to block iC3b binding to its receptor, Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1) |
| Purification method: |
Ion exchange Chromatography |
| Form/Appearance: |
0, 09% sodium azide, PBS |
| Concentration: |
1 miligram per 1 mililiter |
| Storage and shipping: |
Cycles of freezing/defrosting can damage the peptide chains of the antibodies thus reducing the activity, Short term storage of the antibody can be done at +4 degrees Celsius, keep the antibody at -20 degrees Celsius, For long term storage |
| Tested applications:: |
Flow Cytometry (FC/FACS), IHC - Frozen), Immunoprecipitation (IP), Immunohistochemistry (IHC - Paraffin |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by MBS mono they should be stored frozen at - 24° |
| About: |
Each murine monoclonal anibody has his own affinity specific for the clone, Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes, Rats are used to make rat monoclonal anti mouse antibodies, Rats , There are less rat- than mouse clones however, Monoclonals of this antigen are available in different clones, Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats, genes from rodents , of the genus |
| Test: |
A/J, BALB/c, Mouse are mature after 40 days for females and 55 days for males, SCID while the CD-1 is outbred as strain, The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year, Transgenic, congenic and inbread strains are known for C57BL/6, knock-out, Mouse , mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab, or |
| Latin name: |
Rattus norvegicus, Mus musculus |
| Gene target: |
15] (IgG2b) ITGAM / CD11b, Monoclonal [ M1/70 |
| Short name: |
15] (IgG2b) Mouse ITGAM / CD11b Antibody, Monoclonal [clone M1/70 |
| Technique: |
Monoclonals or monoclonal antibodies, antibodies against human proteins, antibodies for, mouses, Antibody |
| Host: |
Rat |
| Isotype: |
IgG2b |
| Species: |
Mouses, Rats, Mouse |
| Alternative name: |
a M (complement component 3 receptor 3 subunit) / CD11b (Antibody to), 15] (IgG2b) to Mouse integrin, Rat monoclonal [clonality M1/70 |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, CD11B and CR3A and MAC-1 and MAC1A and MO1A and SLEB6, ITGAM and IDBG-28139 and ENSG00000169896 and 3684, Itgam and IDBG-210531 and ENSMUSG00000030786 and 16409, alpha M (complement component 3 receptor 3 subunit), nuclei, protein heterodimerization activity, this GO :0001846 and opsonin binding and molecular function this GO :0001948 and glycoprotein binding and molecular function this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002523 and leukocyte migration involved in inflammatory response and biological process this GO :0005515 and protein binding and molecular function this GO :0005634 and nucleus and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0007155 and cell adhesion and biological process this GO :0007159 and leukocyte cell-cell adhesion and biological process this GO :0007229 and integrin-mediated signaling pathway and biological process this GO :0007596 and blood coagulation and biological process this GO :0008201 and heparin binding and molecular function this GO :0008305 and integrin complex and cellular component this GO :0009897 and external side of plasma membrane and cellular component this GO :0009986 and cell surface and cellular component this GO :0014005 and microglia development and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016337 and single organismal cell-cell adhesion and biological process this GO :0030198 and extracellular matrix organization and biological process this GO :0030593 and neutrophil chemotaxis and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0043395 and heparan sulfate proteoglycan binding and molecular function this GO :0045087 and innate immune response and biological process this GO :0045123 and cellular extravasation and biological process this GO :0046872 and metal ion binding and molecular function this GO :0046982 and protein heterodimerization activity and molecular function this GO :0050798 and activated T cell proliferation and biological process this GO :0050900 and leukocyte migration and biological process this GO :0070062 and extracellular vesicular exosome and cellular component, this GO :0001846 : opsonin binding, this GO :0001846 : opsonin binding and also this GO :0001948 : glycoprotein binding and also this GO :0005515 : protein binding and also this GO :0008201 : heparin binding and also this GO :0043395 : heparan sulfate proteoglycan binding and also this GO :0046872 : metal ion binding and also this GO :0046982 : protein heterodimerization activity, this GO :0001948 : glycoprotein binding, this GO :0005515 : protein binding, this GO :0008201 : heparin binding, this GO :0043395 : heparan sulfate proteoglycan binding, this GO :0046872 : metal ion binding, this GO :0046982 : protein heterodimerization activity, 35830 and IDBG-639593 and ENSBTAG00000047238 and 407124, integrin |
| Identity: |
6149 |
| Gene: |
ITGAM |
More about : ITGAM |
| Long gene name: |
integrin subunit alpha M |
| Synonyms gene: |
CR3A CD11B |
| Synonyms gene name: |
alpha, alpha M (complement component 3 receptor 3 subunit) , alpha M (complement component receptor 3, also known as CD11b (p170), integrin, macrophage antigen alpha polypeptide) integrin |
| Synonyms: |
MAC-1 CD11b |
| Synonyms name: |
complement component 3 receptor 3 subunit |
| Locus: |
16p11, 2 |
| Discovery year: |
1988-08-05 |
| GenBank acession: |
J03925 |
| Entrez gene record: |
3684 |
| RefSeq identity: |
NM_000632 |
| Classification: |
Complement system Integrin alpha subunits CD molecules |
| Havana BLAST/BLAT: |
OTTHUMG00000176612 |