| Disease: |
Antibodies and ELISA test kits for detection of listeria antigens, It is usually the causative agent of the relatively rare bacterial disease , The disease affects , The major , adults with , and non , and the , facultatively anaerobic, listeriosis a serious , monocytogenes,  ,  , L, Listeria monocytogenes and , Listeria , Listeria , caused by eating food , contaminated , elderly, genus is , gram-positive, human pathogen , in the , infection , listeriotic, newborns, pregnant women, rod-shaped, species are , spore-forming, weakened immune systems, with the bacteria |
| Technique: |
For real time PCR the cycle threshold Ct values willneed to be set before the experiment, Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment, The , (PCR) amplifies the DNA in your sample, PCR, polymerase chain reaction  |