| Long name: |
Biotin Conjugated, GRB 14 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
GRB 14 |
| Specificity: |
This antibody may also detect the similar protein GRB10 due to an 83% sequence similarity in the immunogen sequence, This is a highly specific antibody against GRB 14 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
220-270/540 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human GRB 14 |
| Gene ID number: |
2888 |
| Swiss Prot: |
Q14449 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Binds to, Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, Potent inhibitor of insulin-stimulated MAPK3 phosphorylation, and suppresses signals from, the activated insulin receptor (INSR), thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal, Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
GRB14, GRB14 adapter protein, Growth factor receptor-bound protein 14 |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
GRB 14 Conjugated |
| Short name: |
Conjugated, GRB 14 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, GRB 14 (Antibody to) |
| Alternative technique: |
antibodies |