| Long name: |
FITC Conjugated, EED Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
EED |
| Specificity: |
This is a highly specific antibody against EED |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human EED |
| Gene ID number: |
8726 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
5% identical to the mouse EED protein and contains seven WD repeats, 535 and 494 amino acids in length, EED (embryonic ectoderm development) is the human homolog of Eed, EED interacts in a highly specific manner, PcG proteins assemble into multimeric protein complexes, The human EED protein is 99, There are two human EED transcripts that contain a putative 407-nucleotide-long intron and give rise to two HEED protein isoforms, a murine PcG gene homologous to the Drosophila homeotic gene, both in vitro and in vivo, extra sex combs, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations, which are involved in protein-protein interactions, with histone deacetylase (HDAC) proteins, The transcriptional repressing Polycomb-group (PcG) and transcriptional activating trithorax-group (trxG) genes of Drosophila are part of a cellular memory system responsible for the stable inheritance of gene activity |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
WAIT-1 HEED |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
EED Conjugated |
| Short name: |
FITC Conjugated, EED Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, EED (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
3188 |
| Gene: |
EED |
More about : EED |
| Long gene name: |
embryonic ectoderm development |
| Synonyms name: |
WD protein associating with integrin cytoplasmic tails 1 |
| Locus: |
11q14, 2 |
| Discovery year: |
1998-12-09 |
| GenBank acession: |
AF078933 |
| Entrez gene record: |
8726 |
| Pubmed identfication: |
9765275 9806832 |
| RefSeq identity: |
NM_003797 |
| Classification: |
Polycomb repressive complex 2 WD repeat domain containing |
| Havana BLAST/BLAT: |
OTTHUMG00000167209 |