| Long name: |
FITC Conjugated, CTRB1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
CTRB1 |
| Specificity: |
This is a highly specific antibody against CTRB1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Chymotrypsin B chain B |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Chymotrypsin B (CTRB1) and Chymotrypsin B2 (CTRB2) are synthesized in the pancreas by protein biosynthesis as precursors that are enzymatically inactive, Chymotrypsins cleave peptide bonds by attacking the non-reactive carbonyl group with a powerful nucleophile, although over time they can also hydrolyze other amide bonds, but become active as three polypeptide molecules that are interconnected by disulfide bonds, especially those with leucine-donated carboxyls, leucine and phenylalanine, tryptophan, which momentarily becomes covalently bonded to the substrate to form an intermediate, Chymotrypsins are digestive enzymes that can perform proteolysis by cleaving peptides at the carboxyl side of tyrosine |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
CTRB1 Conjugated |
| Short name: |
FITC Conjugated, CTRB1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, CTRB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
2521 |
| Gene: |
CTRB1 |
More about : CTRB1 |
| Long gene name: |
chymotrypsinogen B1 |
| Synonyms gene: |
CTRB |
| Locus: |
16q23, 1 |
| Discovery year: |
1986-01-01 |
| Entrez gene record: |
1504 |
| Pubmed identfication: |
2917002 8186414 |
| RefSeq identity: |
NM_001906 |
| Havana BLAST/BLAT: |
OTTHUMG00000159272 |