| Long name: |
FITC Conjugated, BOB1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
BOB1 |
| Specificity: |
This is a highly specific antibody against BOB1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human BOB1 |
| Gene ID number: |
5450 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
A novel protein designated Bob 1 (B cell Oct binding protein 1), ATTTGCAT, Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2, Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2, NF-A1, Oct-1 encodes one protein, Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, The gene which encodes Bob 1 maps to human chromosome 11q23, The versatility of POU protein operation is additionally conferred at the dimerization level, This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes, alternatively called OBF-1, and the proteins that bind to it, could recruit the transcriptional coactivator OBF1, enhancing their transcriptional efficacy, or PORE (ATTTGAAATGCAAAT), specifically interacts with Oct-1 and Oct-2, which is encoded by Oct-2, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, 1, POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
OBF1 BOB1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
BOB1 Conjugated |
| Short name: |
FITC Conjugated, BOB1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, BOB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9211 |
| Gene: |
POU2AF1 |
More about : POU2AF1 |
| Long gene name: |
POU class 2 associating factor 1 |
| Synonyms gene name: |
POU domain class 2, associating factor 1 |
| Locus: |
11q23, 1 |
| Discovery year: |
1995-11-08 |
| Entrez gene record: |
5450 |
| Pubmed identfication: |
8617501 |
| RefSeq identity: |
NM_006235 |
| Havana BLAST/BLAT: |
OTTHUMG00000166659 |