| Long name: |
BOB1 Primary Polyclonal Antibody |
| Also known as: |
BOB1 PAb |
| Category: |
Primary Antibodies |
| Conjugation: |
Unconjugated |
| Target Antigen: |
BOB1 |
| Specificity: |
This is a highly specific antibody against BOB1 |
| Modification(s): |
None |
| Modification site(s): |
Unmodified antibody |
| Clonality: |
Polyclonal antibody |
| Clone number: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human BOB1 |
| Gene ID number: |
5450 |
| Tested Applications: |
IF(IHC-P), IHC-P, WB |
| Recommended dilutions: |
IF(IHC-P)(1:50-200), IHC-P(1:100-500), WB(1:100-1000) |
| Cross reactivity: |
Mouse, Rat, Human |
| Cross reactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Background of the target antigen: |
A novel protein designated Bob 1 (B cell Oct binding protein 1), ATTTGCAT, Although having no intrinsic capacity for DNA binding, Bob 1 associates tightly with the octamer motif in the presence of Oct-1 and/or Oct-2, Bob 1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct co-factor capable of conferring cell-specific activation of Oct-1 and Oct-2, NF-A1, Oct-1 encodes one protein, Studies of tissue-specific expression of immunoglobulin promoters demonstrate the importance of an octamer, The POU dimer from the OCT1 gene formed on the palindromic OCT factor recognition element, The gene which encodes Bob 1 maps to human chromosome 11q23, The versatility of POU protein operation is additionally conferred at the dimerization level, This is a regulatory element important for tissue- and cell-specific transcription as well as for transcription of a number of housekeeping genes, alternatively called OBF-1, and the proteins that bind to it, could recruit the transcriptional coactivator OBF1, enhancing their transcriptional efficacy, or PORE (ATTTGAAATGCAAAT), specifically interacts with Oct-1 and Oct-2, which is encoded by Oct-2, which is found in nuclear extracts from all cell types and thus is not specific to lymphoid cells as is the protein NF-A2, 1, POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store at -20°, 09% sodium azide, C for up to 1 year, Keep the antibody in aqueous buffered solution containing 1% BSA |
| Synonym names: |
B cell specic coactivator OBF 1, B cell specic coactivator OBF1, B-cell-specic coactivator OBF-1, BOB 1, BOB-1, OBF 1, OBF1, OBF1_HUMAN, OCA B, OCA-B, OCAB, OCT binding factor 1, OCT-binding factor 1, POU domain class 2 associating factor 1, POU domain class 2-associating factor 1, POU2AF1, B cell Oct binding protein 1 |
| Properties: |
C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
BOB1 |
| Short name: |
BOB1 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, Antibody |
| Alternative name: |
BOB1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
9211 |
| Gene: |
POU2AF1 |
More about : POU2AF1 |
| Long gene name: |
POU class 2 associating factor 1 |
| Synonyms gene name: |
POU domain class 2, associating factor 1 |
| Synonyms: |
OBF1 BOB1 |
| Locus: |
11q23, 1 |
| Discovery year: |
1995-11-08 |
| Entrez gene record: |
5450 |
| Pubmed identfication: |
8617501 |
| RefSeq identity: |
NM_006235 |
| Havana BLAST/BLAT: |
OTTHUMG00000166659 |