| Long name: |
FITC Conjugated, Blood Group Lewis a Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Blood Group Lewis a |
| Specificity: |
This is a highly specific antibody against Blood Group Lewis a |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human Blood Group Lewis a |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Blood Group Lewis a is a carbohydrate determinant carried on both glycolipids and glycoproteins, Fucosyltransferases catalyze the covalent association of fucose to different positional linkages in sugar acceptor molecules, Hematopoietic lineages rely on Fucosyltransferases to confer a surface carbohydrate phenotype, The carbohydrate moieties generated and covalently attached to cell surfaces are necessary to ensure a surface contour that satisfies physiological roles, determine cell surface-carbohydrate profiles, such as the fucosyltransferases, which are reliant on adhesion molecules such as Selectins (1-3), which is an essential interface for biological recognition processes, which mediates proper cell adhesion molecule recruitment and cell trafficking (4-6), Glycosyltransferases that mediate the regio- and stereoselective transfer of sugars |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
Lewis A antigen |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Group Lewis a Conjugated |
| Short name: |
FITC Conjugated, Blood Group Lewis a Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, Blood family Lewis a (Antibody to) |
| Alternative technique: |
antibodies |