| Long name: |
FITC Conjugated, SP6 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
SP6 |
| Specificity: |
This is a highly specific antibody against SP6 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human SP6 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
EPIPROFIN or KLF14, Expressed ubiquitously with higher expression in developing teeth, Human Sp6 shares 96% sequence homology with its mouse counterpart, Sp6, Sp6 functions to bind GC-rich sequences and related GT and CACCC boxes, The gene encoding Sp6 maps to human chromosome 17, also known as EPFN, hair follicles and limb buds, is a 376 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers, most of which encompass some form of transcriptional activation or repression, suggesting a conserved role between species, thereby promoting cellular proliferation, which comprises over 2, 200 genes, 5% of the human genome and encodes over 1, Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
KLF14 Epfn |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
SP6 Conjugated |
| Short name: |
FITC Conjugated, SP6 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, SP6 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
14530 |
| Gene: |
SP6 |
More about : SP6 |
| Long gene name: |
Sp6 transcription factor |
| Synonyms name: |
epiprofin |
| Locus: |
17q21, 32 |
| Discovery year: |
2001-02-06 |
| Entrez gene record: |
80320 |
| Pubmed identfication: |
11087666 14551215 |
| RefSeq identity: |
NM_199262 |
| Classification: |
Sp transcription factors Zinc fingers C2H2-type |
| Havana BLAST/BLAT: |
OTTHUMG00000132067 |