| Long name: |
FITC Conjugated, B Raf (Thr599) Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
B Raf Thr599 |
| Specificity: |
This is a highly specific antibody against B Raf Thr599 |
| Modification: |
Phosphorylation |
| Modification site: |
Thr599 |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic phosphopeptide derived from human B-Raf around the phosphorylation site of Thr599 |
| Gene ID number: |
673 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
May play a role in the postsynaptic responses of hippocampal neuron, Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway, Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
BRAF, BRAF1, Proto-oncogene B-Raf, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1, Serine/threonine-protein kinase B-raf |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
B Raf (Thr599) Conjugated |
| Short name: |
FITC Conjugated, B Raf (Thr599) Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, B Raf (Thr599) (Antibody to) |
| Alternative technique: |
antibodies |