| Long name: |
FITC Conjugated, ARF1 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
ARF1 |
| Specificity: |
This is a highly specific antibody against ARF1 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Source: |
KLH conjugated synthetic peptide derived from human ARF1 |
| Gene ID number: |
375 |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, In its GTP-bound form, Involved in protein trafficking among different compartments, Modulates vesicle budding and uncoating within the Golgi complex, The hydrolysis of ARF1-bound GTP, an ADP-ribosyltransferase, is required for dissociation of coat proteins from Golgi membranes and vesicles, its triggers the association with coat proteins with the Golgi membrane, suggesting a crucial role in protein trafficking, which is mediated by ARFGAPs proteins, GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
ARF1 Conjugated |
| Short name: |
FITC Conjugated, ARF1 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, ARF1 (Antibody to) |
| Alternative technique: |
antibodies |
| Identity: |
652 |
| Gene: |
ARF1 |
More about : ARF1 |
| Long gene name: |
ADP ribosylation factor 1 |
| Locus: |
1q42, 13 |
| Discovery year: |
1992-07-09 |
| GenBank acession: |
M84326 |
| Entrez gene record: |
375 |
| Pubmed identfication: |
1577740 |
| RefSeq identity: |
NM_001024227 |
| Classification: |
ARF GTPase family Endogenous ligands |
| Havana BLAST/BLAT: |
OTTHUMG00000037595 |