| Long name: |
Biotin Conjugated, Robo3 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Biotinylated |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
Robo3 |
| Specificity: |
This is a highly specific antibody against Robo3 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Extracellular |
| Source: |
KLH conjugated synthetic peptide derived from human Robo3 |
| Tested applications: |
IHC-P, WB |
| Recommended dilutions: |
IHC-P(1:100-500), WB(1:100-1000) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Alternative transcript variants have been described but have not been experimentally validated, CC2, In mouse, Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS), ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily, ROBO3, ROBO3 lacks motif CC1, SLIT proteins 1-3, The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube, This gene, Unlike other ROBO family members, a family of secreted chemorepellants, a transmembrane segment, an autosomal recessive disorder characterized by congenital absence of horizontal gaze, and CC3 (CC for conserved cytoplasmic), and a cytoplasmic tail with three conserved signaling motifs: CC0, and axon fasciculation, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla, and vasculogenesis in addition to neurogenesis, angiogenesis, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, growth cone guidance, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, kidney morphogenesis, leukocyte migration, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain, progressive scoliosis, This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Synonyms: |
HGPS, RB inhibiting gene 1, ROBO3_HUMAN, Rbig 1, Rbig1, Retinoblastoma inhibiting gene 1, Rig 1, Rig1, Robo 3, Robo3, Robo3 protein, Roundabout, Roundabout axon guidance receptor homolog 3, Roundabout homolog 3, Roundabout like protein 3, Roundabout-like protein 3, axon guidance receptor, homolog 3 Drosophila, HGPPS |
| Properties: |
Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA, Biotin conjugates can be detected by horseradish peroxidase, To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1, alkaline phosphatase substrates or anti biotin conjugated antibodies, 6, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
Robo3 Conjugated |
| Short name: |
Conjugated, Robo3 Antibody |
| Technique: |
antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
Biotin |
| Alternative name: |
biotinilated coupled, Robo3 (Antibody to) |
| Alternative technique: |
antibodies |