| Long name: |
FITC Conjugated, TLR4 Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
TLR4 |
| Specificity: |
This is a highly specific antibody against TLR4 |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Immunogen_range: |
780-830/835 |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from rat TLR4 |
| Gene ID number: |
21898 |
| Swiss Prot: |
Q9QUK6 |
| Tested applications: |
IF(ICC), IF(IHC-P), FCM |
| Recommended dilutions: |
IF(ICC)(1:50-200), IF(IHC-P)(1:50-200), FCM(1:20-100) |
| Crossreactivity: |
Bovine, Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
Acts via MYD88, Also involved in LPS-independent inflammatory responses triggered by free fatty acids, CXCL2 and CCL9 cytokines, In complex with TLR6, In this context, TIRAP and TRAF6, This event induces the formation of a heterodimer of TLR4 and TLR6, and CCL5 cytokine, as well as IL1B secretion, cytokine secretion and the inflammatory response, leading to NF-kappa-B activation, leading to the NF-kappa-B-dependent production of CXCL1, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42, such as palmitate, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36, via MYD88 signaling pathway, via TICAM1 signaling pathway, which is rapidly internalized and triggers inflammatory response, Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
hToll CD284 TLR-4 ARMD10 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
TLR4 Conjugated |
| Short name: |
FITC Conjugated, TLR4 Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, toll-like receptor 4 (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
ARMD10 and CD284 and TLR-4 and TOLL, Cell surfaces, TLR4 and IDBG-638758 and ENSBTAG00000006240 and 281536, TLR4 and IDBG-82738 and ENSG00000136869 and 7099, Tlr4 and IDBG-156729 and ENSMUSG00000039005 and 21898, protein binding, this GO :0000187 and activation of MAPK activity and biological process this GO :0001530 and lipopolysaccharide binding and molecular function this GO :0001875 and lipopolysaccharide receptor activity and molecular function this GO :0002218 and activation of innate immune response and biological process this GO :0002224 and toll-like receptor signaling pathway and biological process this GO :0002322 and B cell proliferation involved in immune response and biological process this GO :0002537 and nitric oxide production involved in inflammatory response and biological process this GO :0002755 and MyD88-dependent toll-like receptor signaling pathway and biological process this GO :0002756 and MyD88-independent toll-like receptor signaling pathway and biological process this GO :0004872 and receptor activity and molecular function this GO :0004888 and transmembrane signaling receptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005737 and cytoplasm and cellular component this GO :0005886 and plasma membrane and cellular component this GO :0005887 and integral component of plasma membrane and cellular component this GO :0006955 and immune response and biological process this GO :0007165 and signal transduction and biological process this GO :0007249 and I-kappaB kinase/NF-kappaB signaling and biological process this GO :0007252 and I-kappaB phosphorylation and biological process this GO :0009617 and response to bacterium and biological process this GO :0009897 and external side of plasma membrane and cellular component this GO :0010008 and endosome membrane and cellular component this GO :0010572 and positive regulation of platelet activation and biological process this GO :0016021 and integral component of membrane and cellular component this GO :0016046 and detection of fungus and biological process this GO :0030890 and positive regulation of B cell proliferation and biological process this GO :0031663 and lipopolysaccharide-mediated signaling pathway and biological process this GO :0032496 and response to lipopolysaccharide and biological process this GO :0032497 and detection of lipopolysaccharide and biological process this GO :0032609 and interferon-gamma production and biological process this GO :0032689 and negative regulation of interferon-gamma production and biological process this GO :0032700 and negative regulation of interleukin-17 production and biological process this GO :0032707 and negative regulation of interleukin-23 production and biological process this GO :0032715 and negative regulation of interleukin-6 production and biological process this GO :0032720 and negative regulation of tumor necrosis factor production and biological process this GO :0032722 and positive regulation of chemokine production and biological process this GO :0032727 and positive regulation of interferon-alpha production and biological process this GO :0032728 and positive regulation of interferon-beta production and biological process this GO :0032729 and positive regulation of interferon-gamma production and biological process this GO :0032732 and positive regulation of interleukin-1 production and biological process this GO :0032733 and positive regulation of interleukin-10 production and biological process this GO :0032735 and positive regulation of interleukin-12 production and biological process this GO :0032755 and positive regulation of interleukin-6 production and biological process this GO :0032757 and positive regulation of interleukin-8 production and biological process this GO :0032760 and positive regulation of tumor necrosis factor production and biological process this GO :0032874 and positive regulation of stress-activated MAPK cascade and biological process this GO :0034134 and toll-like receptor 2 signaling pathway and biological process this GO :0034138 and toll-like receptor 3 signaling pathway and biological process this GO :0034142 and toll-like receptor 4 signaling pathway and biological process this GO :0035666 and TRIF-dependent toll-like receptor signaling pathway and biological process this GO :0038123 and toll-like receptor TLR1:TLR2 signaling pathway and biological process this GO :0038124 and toll-like receptor TLR6:TLR2 signaling pathway and biological process this GO :0042088 and T-helper 1 type immune response and biological process this GO :0042116 and macrophage activation and biological process this GO :0042346 and positive regulation of NF-kappaB import into nucleus and biological process this GO :0042535 and positive regulation of tumor necrosis factor biosynthetic process and biological process this GO :0042742 and defense response to bacterium and biological process this GO :0043123 and positive regulation of I-kappaB kinase/NF-kappaB signaling and biological process this GO :0045084 and positive regulation of interleukin-12 biosynthetic process and biological process this GO :0045087 and innate immune response and biological process this GO :0045348 and positive regulation of MHC class II biosynthetic process and biological process this GO :0045359 and positive regulation of interferon-beta biosynthetic process and biological process this GO :0045416 and positive regulation of interleukin-8 biosynthetic process and biological process this GO :0045429 and positive regulation of nitric oxide biosynthetic process and biological process this GO :0045671 and negative regulation of osteoclast differentiation and biological process this GO :0045944 and positive regulation of transcription from RNA polymerase II promoter and biological process this GO :0046330 and positive regulation of JNK cascade and biological process this GO :0046696 and lipopolysaccharide receptor complex and cellular component this GO :0048471 and perinuclear region of cytoplasm and cellular component this GO :0050671 and positive regulation of lymphocyte proliferation and biological process this GO :0050707 and regulation of cytokine secretion and biological process this GO :0050727 and regulation of inflammatory response and biological process this GO :0050729 and positive regulation of inflammatory response and biological process this GO :0050829 and defense response to Gram-negative bacterium and biological process this GO :0051092 and positive regulation of NF-kappaB transcription factor activity and biological process this GO :0051770 and positive regulation of nitric-oxide synthase biosynthetic process and biological process this GO :0060729 and intestinal epithelial structure maintenance and biological process this GO :0060907 and positive regulation of macrophage cytokine production and biological process this GO :0070373 and negative regulation of ERK1 and ERK2 cascade and biological process this GO :0070374 and positive regulation of ERK1 and ERK2 cascade and biological process this GO :0070430 and positive regulation of nucleotide-binding oli this GO merization domain containing 1 signaling pathway and biological process this GO :0070434 and positive regulation of nucleotide-binding oli this GO merization domain containing 2 signaling pathway and biological process this GO :0071222 and cellular response to lipopolysaccharide and biological process this GO :0071223 and cellular response to lipoteichoic acid and biological process this GO :0071260 and cellular response to mechanical stimulus and biological process, this GO :0001530 : lipopolysaccharide binding, this GO :0001530 : lipopolysaccharide binding and also this GO :0001875 : lipopolysaccharide receptor activity and also this GO :0004872 : receptor activity and also this GO :0004888 : transmembrane signaling receptor activity and also this GO :0005515 : protein binding, this GO :0001875 : lipopolysaccharide receptor activity, this GO :0004872 : receptor activity, this GO :0004888 : transmembrane signaling receptor activity, this GO :0005515 : protein binding, toll-like receptor 4 |
| Identity: |
11850 |
| Gene: |
TLR4 |
More about : TLR4 |
| Long gene name: |
toll like receptor 4 |
| Synonyms gene name: |
toll-like receptor 4 |
| Locus: |
9q33, 1 |
| Discovery year: |
1998-06-25 |
| GenBank acession: |
U88880 |
| Entrez gene record: |
7099 |
| Pubmed identfication: |
9435236 9237759 |
| RefSeq identity: |
NM_138554 |
| Classification: |
CD molecules Toll like receptors |
| Havana BLAST/BLAT: |
OTTHUMG00000021046 |