| Long name: |
FITC Conjugated, LAT Polyclonal Antibody |
| Category: |
Conjugated Primary Antibodies |
| Conjugation: |
Anti-FITC Antibody |
| Host Organism: |
Rabbit (Oryctolagus cuniculus) |
| Target Antigen: |
LAT |
| Specificity: |
This is a highly specific antibody against LAT |
| Modification: |
Unmodified |
| Modification site: |
None |
| Clonality: |
Polyclonal |
| Clone: |
Polyclonal antibody |
| Concentration: |
1ug per 1ul |
| Subcellular location: |
Cytoplasm |
| Source: |
KLH conjugated synthetic peptide derived from human LAT |
| Tested applications: |
IF(IHC-P) |
| Recommended dilutions: |
IF(IHC-P)(1:50-200) |
| Crossreactivity: |
Mouse, Rat, Human |
| Crossreactive species details: |
not every possible cross-reactivity is known, Due to limited amount of testing and knowledge |
| Antigen background: |
LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen, LAT is expressed in T lymphocytes in interstitial spaces, LAT is not expressed in B cells, epithelial histiocytes and dendritic cells, macrophages, monocytes, plasma cells, platelets and megakaryocytes, which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation, Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD) |
| Purification method: |
This antibody was purified via Protein A |
| Storage conditions: |
50% glycerol and 0, Store refrigerated at 2 to 8 degrees Celcius for up to 1 year, 09% sodium azide, Keep the antibody in an aqueous buffered solution containing 1% BSA |
| Excitation Emission: |
494nm/518nm |
| Synonyms: |
LAT1 |
| Properties: |
Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes, FITC has a green emission that's usually collected at 530 nanometers, FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light, For fluorescent microscopy applications, This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS, When excited at 488 nanometers, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, the FL1 detector of a FACSCalibur or FACScan, C, C for long term storage and for short term at + 5°, If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24° |
| Gene target: |
LAT Conjugated |
| Short name: |
FITC Conjugated, LAT Antibody |
| Technique: |
Fluorescein, antibodies against human proteins, antibodies for, antibody Conjugates, Antibody |
| Label: |
FITC |
| Alternative name: |
fluorecein coupled, linker for activation on T cells (Antibody to) |
| Alternative technique: |
antibodies |
| Alternative to gene target: |
BT, Cell surfaces, LAT and IDBG-239499 and ENSG00000213658 and 27040, Lat and IDBG-209436 and ENSMUSG00000030742 and 16797, protein kinase binding, this GO :0001772 and immunological synapse and cellular component this GO :0002260 and lymphocyte homeostasis and biological process this GO :0005070 and SH3/SH2 adaptor activity and molecular function this GO :0005515 and protein binding and molecular function this GO :0005886 and plasma membrane and cellular component this GO :0005911 and cell-cell junction and cellular component this GO :0006954 and inflammatory response and biological process this GO :0006955 and immune response and biological process this GO :0007165 and signal transduction and biological process this GO :0007229 and integrin-mediated signaling pathway and biological process this GO :0007265 and Ras protein signal transduction and biological process this GO :0007596 and blood coagulation and biological process this GO :0008180 and COP9 signalosome and cellular component this GO :0009967 and positive regulation of signal transduction and biological process this GO :0010467 and gene expression and biological process this GO :0016020 and membrane and cellular component this GO :0016021 and integral component of membrane and cellular component this GO :0019722 and calcium-mediated signaling and biological process this GO :0019901 and protein kinase binding and molecular function this GO :0030168 and platelet activation and biological process this GO :0035556 and intracellular signal transduction and biological process this GO :0038095 and Fc-epsilon receptor signaling pathway and biological process this GO :0042110 and T cell activation and biological process this GO :0042629 and mast cell granule and cellular component this GO :0043303 and mast cell degranulation and biological process this GO :0045087 and innate immune response and biological process this GO :0045121 and membrane raft and cellular component this GO :0048872 and homeostasis of number of cells and biological process this GO :0050852 and T cell receptor signaling pathway and biological process this GO :0050863 and regulation of T cell activation and biological process, this GO :0005070 : SH3/SH2 adaptor activity, this GO :0005070 : SH3/SH2 adaptor activity and also this GO :0005515 : protein binding and also this GO :0019901 : protein kinase binding, this GO :0005515 : protein binding, this GO :0019901 : protein kinase binding, 26847 and IDBG-638875 and ENSBTAG00000021249 and 514735, linker for activation of T cells |
| Identity: |
18874 |
| Gene: |
LAT |
More about : LAT |
| Long gene name: |
linker for activation of T-cells |
| Synonyms gene name: |
linker for activation of T cells |
| Synonyms name: |
linker for activation of T cells, transmembrane adaptor |
| Locus: |
16q13 |
| Discovery year: |
2002-07-12 |
| GenBank acession: |
AF036905 |
| Pubmed identfication: |
9489702 |
| Havana BLAST/BLAT: |
OTTHUMG00000131761 |